HEK-Blue-Lucia hTLR5; HEK-Dual hTLR5 (NF/IL8)细胞株NTCC® Cell Line BioVector细胞保藏中心
- 价 格：¥99850
- 货 号：HEK-Blue-Lucia hTLR5; HEK-Dual hTLR5 (NF/IL8)
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
[Designation名称] NTCC® HEK-Blue-Lucia hTLR5; HEK-Dual hTLR5 (NF/IL8) Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: ID HEK-Blue-Lucia hTLR5 (NF/IL8)Cross-references: Wikidata; Q107115292Accession Number: NTCC® CVCL_A8CTCross-references: InvivoGen; hkd-htlr5niComments: Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia (InvivoGen=hkd-htlr5ni).Comments: Knockout cell: Method=Homologous recombination; HGNC; HGNC:6025; CXCL8.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A.Comments: Genetic integration: Method=Homologous recombination; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11851; TLR5.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_A8CT
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK-Blue-Lucia hTLR5; HEK-Dual hTLR5 (NF/IL8) Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: ID HEK-Blue-Lucia hTLR5 (NF/IL8)Cross-references: Wikidata; Q107115292Accession Number: NTCC® CVCL_A8CTCross-references: InvivoGen; hkd-htlr5niComments: Characteristics: Stably expresses two reporter genes: SEAP (secreted ALPP) and the Lucia luciferase. In this cell line SEAP is under the control of an IFN-beta minimal promoter fused to 5 NF-kappaB and AP-1-binding sites. The Lucia luciferase reporter gene expression depends on the activation of the endogenous CXCL8/IL8 promoter as the CXCL8 gene has been replaced by Lucia (InvivoGen=hkd-htlr5ni).Comments: Knockout cell: Method=Homologous recombination; HGNC; HGNC:6025; CXCL8.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11849; TLR3.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11851; TLR5.Comments: Knockout cell: Method=Not specified; HGNC; HGNC:11916; TNFRSF1A.Comments: Genetic integration: Method=Homologous recombination; Gene=Synthetic construct Lucia luciferase (Note=Synthetic gene designed on natural secreted luciferase genes from marine copepods).Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:439; ALPP.Comments: Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11851; TLR5.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P33967; Bacillus cereus Bsr.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC® CVCL_A8CT
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK-Blue-Lucia hTLR5; HEK-Dual hTLR5 (NF/IL8) Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
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