MB135-iDUX4人端粒酶永生化细胞株BioVector NTCC细胞保藏中心

[Designation名称] NTCC® MB135-iDUX4 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: MB135-iDUX4 clone #7
References identifiers: PubMed=28171552;
Comments: Characteristics: Transduced with plasmid pCW57.1-DUX4-CA that contains a tetO driven codon altered DUX4 and a hPGK-puroR-T2A-rtTA, where reverse tetracycline-controlled transactivator (rtTA) is a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16. Will produces the codon-altered DUX4 in response to induction by doxycycline (PubMed=28171552).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:50800; DUX4 (Note=Codon-altered).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11730; TERT.
Comments: Genetic integration: Method=Transduction; Gene=MGI; MGI:88357; Cdk4.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 (HHV-1) VP16 (UL28) (Note=With p.Met1_Arg360del).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Thigh, quadriceps skeletal muscle; UBERON=UBERON_0004498.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession Number: NTCC® CVCL_F1JH
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® MB135-iDUX4 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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