HBPC/ci37条件永生化人胎脑周细胞系BioVector®人血脑屏障(BBB)体外模型 BioVector NTCC保藏中心
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- 货 号：BioVector®-HBPC/ci37
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- BioVector NTCC典型培养物保藏中心
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电话：400-800-2947 工作QQ:1843439339 (微信同号)
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BioVector® NTCC® HBPC/ci37条件永生化人胎脑周细胞系BioVector®人血脑屏障(BBB)体外模型
The HBPC/ci37 cell line is a conditionally immortalized human fetal brain pericyte cell line used primarily as an in vitro model for studying the human blood-brain barrier (BBB). These cells possess key brain pericyte functions while offering the practical advantage of continuous proliferation in a lab setting. Key Characteristics Organism: Homo sapiens (Human) Tissue Source: Fetal brain pericytes Morphology: Exhibits typical features of brain pericytes Key Trait: Conditionally immortalized, meaning their proliferation can be controlled by temperature manipulation. Immortalization Genes: Carries two specific genes for immortalization: Temperature-sensitive simian virus 40 large tumor antigen (tsSV40T): Promotes proliferation at 33°C. Human telomerase reverse transcriptase catalytic subunit (hTERT): Contributes to indefinite lifespan. Research Use: Used in the development of highly relevant human-based tricellular (with endothelial cells and astrocytes) static transwell or 3D "Brain-on-a-Chip" models of the blood-brain barrier for drug permeability studies. Culture Conditions The conditional nature of this cell line means that culture conditions are critical and must be adjusted based on whether proliferation or differentiation is desired: Maintenance (Proliferation): Temperature: Cells should be cultured at 33°C to maintain the active function of the tsSV40T antigen and promote continuous proliferation. Medium Supplement: Blasticidin S (at a final concentration of 4 µg/mL) must always be added to the culture medium to maintain the expression of the immortalization genes. Differentiation (Functional Assays): Temperature: To induce differentiation and form a functional barrier model, the culture temperature should be raised to 37°C. At this temperature, the tsSV40T antigen becomes unstable and loses its function, turning off immortalization signals. Medium Change: For differentiation, the medium is typically replaced with FBS- and blasticidin S-free differentiation medium. HBPC/ci37 细胞系是一种条件永生化人胎脑周细胞系，主要用作研究人血脑屏障 (BBB) 的体外模型。这些细胞具有脑周细胞的关键功能，同时提供了在实验室环境中持续增殖的实用优势。 主要特征 生物体： Homo sapiens (人) 组织来源： 胎脑周细胞 形态： 表现出典型的脑周细胞特征 关键特性： 条件永生化，意味着它们的增殖可以通过温度控制来调节。 永生化基因： 携带两个特定的永生化基因： 温度敏感型猿猴病毒 40 大肿瘤抗原 (tsSV40T)： 在 33°C 下促进增殖。 人端粒酶逆转录酶催化亚基 (hTERT)： 有助于实现无限寿命。 研究用途： 用于开发高度相关的人类血脑屏障三细胞（与内皮细胞和星形胶质细胞共培养）静态 Transwell 或 3D“芯片大脑”模型，以进行药物渗透性研究。 培养条件 该细胞系的条件性质意味着培养条件至关重要，必须根据需要增殖还是分化进行调整： 维持（增殖）： 温度： 细胞应在 33°C 下培养，以维持 tsSV40T 抗原的活性功能并促进持续增殖。 培养基补充剂： 必须始终在培养基中添加杀稻瘟菌素 S（终浓度为 4 µg/mL），以维持永生化基因的表达。 分化（功能测定）： 温度： 为了诱导分化并形成功能性屏障模型，培养温度应升至 37°C。在此温度下，tsSV40T 抗原变得不稳定并失去功能，从而关闭永生化信号。 培养基更换： 为了促进分化，通常将培养基更换为不含 FBS 和杀稻瘟菌素 S 的分化培养基。 研究人员利用这种独特的特性在细胞的快速生长期（用于扩增）和功能成熟期（用于实验测定）之间进行切换。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
The HBPC/ci37 cell line is a conditionally immortalized human fetal brain pericyte cell line used primarily as an in vitro model for studying the human blood-brain barrier (BBB). These cells possess key brain pericyte functions while offering the practical advantage of continuous proliferation in a lab setting. Key Characteristics Organism: Homo sapiens (Human) Tissue Source: Fetal brain pericytes Morphology: Exhibits typical features of brain pericytes Key Trait: Conditionally immortalized, meaning their proliferation can be controlled by temperature manipulation. Immortalization Genes: Carries two specific genes for immortalization: Temperature-sensitive simian virus 40 large tumor antigen (tsSV40T): Promotes proliferation at 33°C. Human telomerase reverse transcriptase catalytic subunit (hTERT): Contributes to indefinite lifespan. Research Use: Used in the development of highly relevant human-based tricellular (with endothelial cells and astrocytes) static transwell or 3D "Brain-on-a-Chip" models of the blood-brain barrier for drug permeability studies. Culture Conditions The conditional nature of this cell line means that culture conditions are critical and must be adjusted based on whether proliferation or differentiation is desired: Maintenance (Proliferation): Temperature: Cells should be cultured at 33°C to maintain the active function of the tsSV40T antigen and promote continuous proliferation. Medium Supplement: Blasticidin S (at a final concentration of 4 µg/mL) must always be added to the culture medium to maintain the expression of the immortalization genes. Differentiation (Functional Assays): Temperature: To induce differentiation and form a functional barrier model, the culture temperature should be raised to 37°C. At this temperature, the tsSV40T antigen becomes unstable and loses its function, turning off immortalization signals. Medium Change: For differentiation, the medium is typically replaced with FBS- and blasticidin S-free differentiation medium. HBPC/ci37 细胞系是一种条件永生化人胎脑周细胞系，主要用作研究人血脑屏障 (BBB) 的体外模型。这些细胞具有脑周细胞的关键功能，同时提供了在实验室环境中持续增殖的实用优势。 主要特征 生物体： Homo sapiens (人) 组织来源： 胎脑周细胞 形态： 表现出典型的脑周细胞特征 关键特性： 条件永生化，意味着它们的增殖可以通过温度控制来调节。 永生化基因： 携带两个特定的永生化基因： 温度敏感型猿猴病毒 40 大肿瘤抗原 (tsSV40T)： 在 33°C 下促进增殖。 人端粒酶逆转录酶催化亚基 (hTERT)： 有助于实现无限寿命。 研究用途： 用于开发高度相关的人类血脑屏障三细胞（与内皮细胞和星形胶质细胞共培养）静态 Transwell 或 3D“芯片大脑”模型，以进行药物渗透性研究。 培养条件 该细胞系的条件性质意味着培养条件至关重要，必须根据需要增殖还是分化进行调整： 维持（增殖）： 温度： 细胞应在 33°C 下培养，以维持 tsSV40T 抗原的活性功能并促进持续增殖。 培养基补充剂： 必须始终在培养基中添加杀稻瘟菌素 S（终浓度为 4 µg/mL），以维持永生化基因的表达。 分化（功能测定）： 温度： 为了诱导分化并形成功能性屏障模型，培养温度应升至 37°C。在此温度下，tsSV40T 抗原变得不稳定并失去功能，从而关闭永生化信号。 培养基更换： 为了促进分化，通常将培养基更换为不含 FBS 和杀稻瘟菌素 S 的分化培养基。 研究人员利用这种独特的特性在细胞的快速生长期（用于扩增）和功能成熟期（用于实验测定）之间进行切换。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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