- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
BioVector® NTCC® A549/DDP人肺腺癌细胞株顺铂耐药亚株
The A549/DDP cell line is a cisplatin-resistant subline of the human lung adenocarcinoma A549 cell line. It was developed through continuous exposure to increasing concentrations of cisplatin (DDP) and is a critical model for studying mechanisms of chemotherapy resistance in lung cancer research. Key Characteristics Organism: Homo sapiens (Human) Tissue: Lung (pulmonary adenocarcinoma) Disease: Non-small cell lung cancer (NSCLC) Morphology: Epithelial-like, adherent monolayer cells, often noted to exhibit a more fibroblastic morphology compared to the parental A549 cells. Growth Properties: Adherent. Key Trait: Exhibits significant resistance to cisplatin-induced cell death; the IC50 value is significantly higher than that of the parental A549 line. Biosafety Level: Typically BSL-1. Research Applications The A549/DDP cell line is primarily used to: Investigate the biochemical pathways and mechanisms that confer resistance to cisplatin (e.g., Nrf2-mediated defenses, c-met pathway, increased autophagy). Screen for new drugs or drug combinations that can reverse resistance and sensitize the cells to cisplatin treatment. Culture Conditions Culture Medium: RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) and stable L-glutamine (2.0 mM) is commonly used. Maintenance: To maintain the drug-resistant phenotype, the cells are typically cultured in medium containing a specific concentration of DDP (e.g., 10μM or 1μM, depending on the specific protocol/supplier). This cisplatin is often withdrawn for a few generations immediately before experiments are conducted. Growth Temperature: 37°C. CO₂ Concentration: 5% CO₂ in a humidified incubator. Subculture Routine: Wash cells with PBS lacking calcium and magnesium. Detachment is typically done using Accutase or trypsin/EDTA solution. Subcultivate when 70-80% confluent. A549/DDP 细胞系是人肺腺癌 A549 细胞系的顺铂耐药亚株。它是通过持续暴露于浓度递增的顺铂 (DDP) 中而培养出来的，是肺癌研究中用于研究化疗耐药机制的关键模型。 主要特征 生物体： Homo sapiens (人) 组织： 肺（肺腺癌） 疾病： 非小细胞肺癌 (NSCLC) 形态： 上皮样，贴壁单层细胞，通常被描述为与亲代 A549 细胞相比具有更强的成纤维细胞样形态。 生长特性： 贴壁生长。 关键特性： 对顺铂诱导的细胞死亡表现出显著抵抗力；IC50 值远高于亲代 A549 细胞系。 生物安全级别： 通常为 BSL-1。 研究应用 A549/DDP 细胞系主要用于： 研究赋予顺铂耐药性的生化途径和机制（例如，Nrf2 介导的防御、c-met 通路、自噬增加）。 筛选可以逆转耐药性并使细胞对顺铂治疗敏感的新药物或药物组合。 培养条件 培养基： 通常使用补充 10% 胎牛血清 (FBS) 和稳定 L-谷氨酰胺 (2.0 mM) 的 RPMI 1640 培养基。 维护： 为了维持耐药表型，通常将细胞培养在含有特定浓度 DDP（例如 10μM 或 1μM，取决于具体的方案/供应商）的培养基中。在进行实验前，通常会立即停止使用顺铂几代。 生长温度： 37°C。 CO₂ 浓度： 在 37°C、5% CO₂ 的加湿培养箱中培养。 传代程序： 用不含钙和镁的 PBS 洗涤细胞。 通常使用 Accutase 或胰蛋白酶/EDTA 溶液进行细胞消化。 当细胞汇合度达到 70-80% 时进行传代。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
The A549/DDP cell line is a cisplatin-resistant subline of the human lung adenocarcinoma A549 cell line. It was developed through continuous exposure to increasing concentrations of cisplatin (DDP) and is a critical model for studying mechanisms of chemotherapy resistance in lung cancer research. Key Characteristics Organism: Homo sapiens (Human) Tissue: Lung (pulmonary adenocarcinoma) Disease: Non-small cell lung cancer (NSCLC) Morphology: Epithelial-like, adherent monolayer cells, often noted to exhibit a more fibroblastic morphology compared to the parental A549 cells. Growth Properties: Adherent. Key Trait: Exhibits significant resistance to cisplatin-induced cell death; the IC50 value is significantly higher than that of the parental A549 line. Biosafety Level: Typically BSL-1. Research Applications The A549/DDP cell line is primarily used to: Investigate the biochemical pathways and mechanisms that confer resistance to cisplatin (e.g., Nrf2-mediated defenses, c-met pathway, increased autophagy). Screen for new drugs or drug combinations that can reverse resistance and sensitize the cells to cisplatin treatment. Culture Conditions Culture Medium: RPMI 1640 medium supplemented with 10% Fetal Bovine Serum (FBS) and stable L-glutamine (2.0 mM) is commonly used. Maintenance: To maintain the drug-resistant phenotype, the cells are typically cultured in medium containing a specific concentration of DDP (e.g., 10μM or 1μM, depending on the specific protocol/supplier). This cisplatin is often withdrawn for a few generations immediately before experiments are conducted. Growth Temperature: 37°C. CO₂ Concentration: 5% CO₂ in a humidified incubator. Subculture Routine: Wash cells with PBS lacking calcium and magnesium. Detachment is typically done using Accutase or trypsin/EDTA solution. Subcultivate when 70-80% confluent. A549/DDP 细胞系是人肺腺癌 A549 细胞系的顺铂耐药亚株。它是通过持续暴露于浓度递增的顺铂 (DDP) 中而培养出来的，是肺癌研究中用于研究化疗耐药机制的关键模型。 主要特征 生物体： Homo sapiens (人) 组织： 肺（肺腺癌） 疾病： 非小细胞肺癌 (NSCLC) 形态： 上皮样，贴壁单层细胞，通常被描述为与亲代 A549 细胞相比具有更强的成纤维细胞样形态。 生长特性： 贴壁生长。 关键特性： 对顺铂诱导的细胞死亡表现出显著抵抗力；IC50 值远高于亲代 A549 细胞系。 生物安全级别： 通常为 BSL-1。 研究应用 A549/DDP 细胞系主要用于： 研究赋予顺铂耐药性的生化途径和机制（例如，Nrf2 介导的防御、c-met 通路、自噬增加）。 筛选可以逆转耐药性并使细胞对顺铂治疗敏感的新药物或药物组合。 培养条件 培养基： 通常使用补充 10% 胎牛血清 (FBS) 和稳定 L-谷氨酰胺 (2.0 mM) 的 RPMI 1640 培养基。 维护： 为了维持耐药表型，通常将细胞培养在含有特定浓度 DDP（例如 10μM 或 1μM，取决于具体的方案/供应商）的培养基中。在进行实验前，通常会立即停止使用顺铂几代。 生长温度： 37°C。 CO₂ 浓度： 在 37°C、5% CO₂ 的加湿培养箱中培养。 传代程序： 用不含钙和镁的 PBS 洗涤细胞。 通常使用 Accutase 或胰蛋白酶/EDTA 溶液进行细胞消化。 当细胞汇合度达到 70-80% 时进行传代。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 A549/DDP人肺腺癌细胞株顺铂耐药亚株 BioVector NTCC保藏中心 的询问
- 公告/新闻
