pSRK广宿主表达载体BioVector®质粒 BioVector NTCC保藏中心

BioVector® NTCC® pSRK广宿主表达载体BioVector®质粒

The pSRK plasmid family consists of broad-host-range expression vectors used for tightly regulated gene expression in diverse Gram-negative bacteria. These plasmids are popular because they can function in a wide range of bacterial species and feature a tightly controlled, IPTG-inducible promoter system. Description and Characteristics Vector Type: Broad-host-range expression vector, meaning it is compatible with many different types of Gram-negative bacteria. Backbone Origin: The design is based on the pBBR1 replicon, which is a stable origin of replication. Host Range: Effective in a wide variety of bacteria, including Agrobacterium tumefaciens, Pseudomonas fluorescens, Rhizobium leguminosarum, and Caulobacter crescentus. Expression System: Uses an IPTG-inducible lac promoter system (lacIq-Plac). This system keeps the target gene expression turned off until the inducer chemical, IPTG, is added to the medium. Selection Marker: The specific antibiotic resistance marker varies depending on the variant of the pSRK plasmid. Common Variants pSRKKm: Confers resistance to kanamycin (typically used at 25-50 µg/mL). pSRKGm: Confers resistance to gentamicin. pSRKTc: Confers resistance to tetracycline. Usage and Culture Conditions Host Organisms: Can be propagated in standard E. coli cloning strains like DH5alpha, but is engineered for functional use in other diverse Gram-negative hosts. Culture Medium: Luria–Bertani (LB) medium is standard for E. coli. The specific requirements for different bacterial hosts may vary. Temperature: Typically cultured at 37°C for E. coli, but some specific hosts like Rhodococcus opacus PD630 grow optimally at 30°C. Induction: Gene expression is switched "on" by adding the chemical IPTG (Isopropyl β-D-1-thiogalactopyranoside) to the actively growing culture medium. pSRK 质粒家族包含广宿主范围的表达载体，用于在多种革兰氏阴性细菌中进行严格调控的基因表达。它们之所以受欢迎，是因为它们能够在多种细菌物种中发挥作用，并且具有严格控制的、IPTG 诱导型启动子系统。 描述和特征 载体类型： 广宿主范围表达载体，意味着它与许多不同类型的革兰氏阴性细菌兼容。 骨架来源： 该设计基于 pBBR1 复制子，这是一个稳定的复制起点。 宿主范围： 在多种细菌中有效，包括根癌农杆菌 (Agrobacterium tumefaciens)、荧光假单胞菌 (Pseudomonas fluorescens)、豌豆根瘤菌 (Rhizobium leguminosarum) 和新月柄杆菌 (Caulobacter crescentus)。 表达系统： 使用 IPTG 诱导型 lac 启动子系统 (lacIq-Plac)。该系统使目标基因的表达保持关闭状态，直到将诱导剂化学物质 IPTG 添加到培养基中。 筛选标记： 特异的抗生素耐药性标记根据 pSRK 质粒的不同变体而异。 常见变体 pSRKKm： 赋予卡那霉素耐药性（通常使用 25-50 µg/mL）。 pSRKGm： 赋予庆大霉素耐药性。 pSRKTc： 赋予四环素耐药性。 用途和培养条件 宿主生物体： 可以在标准大肠杆菌克隆菌株（如 DH5alpha）中繁殖，但设计用于在其他多种革兰氏阴性宿主中发挥功能。 培养基： Luria–Bertani (LB) 培养基是大肠杆菌的标准培养基。不同细菌宿主的具体要求可能有所不同。 温度： 大肠杆菌通常在 37°C 培养，但某些特定宿主（如抗酸罗氏菌 Rhodococcus opacus PD630）在 30°C 下生长最佳。 诱导： 通过向积极生长的培养基中添加化学物质 IPTG（异丙基-β-D-硫代半乳糖苷）来开启基因表达。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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