MM294大肠杆菌 (E. coli) 菌株高产量纯净质粒 DNA生产菌种BioVector® BioVector NTCC保藏中心

BioVector® NTCC® MM294大肠杆菌 (E. coli) 菌株高产量纯净质粒 DNA生产菌种BioVector®

The BioVector® E. coli MM294 strain is a widely used laboratory strain of Escherichia coli, derived from the K-12 lineage. It is primarily employed in cloning and plasmid DNA production because of its high efficiency in taking up foreign DNA (transformability) and its ability to yield high quantities of pure plasmid DNA. Description and Characteristics Organism: Escherichia coli. Strain Designation: K12 MM294, or simply MM294. Origin: It originated from the laboratory of Matthew Meselson. Primary Use: A robust host for the transformation and propagation of plasmids in molecular biology workflows. Phenotype: It has a standard wild-type appearance. Plasmid Status: The strain is free of any native plasmids. Biosafety Level: It is typically classified as Biosafety Level 1 (BSL-1). Key Genetic Features (Genotype) The specific genetic modifications of MM294 provide functional advantages for cloning: F⁻: This means the strain is a recipient during bacterial conjugation, making it unable to act as a donor of genetic material via conjugation. endA1 mutation: This mutation inactivates the cell's internal endonuclease I enzyme. The absence of this enzyme is crucial because it prevents the degradation of delicate plasmid DNA during the purification process, leading to better yields and higher quality DNA. hsdR17 (rK⁻mK⁺) mutation: This affects the restriction-modification system. The strain cannot cut (restrict) foreign DNA that lacks specific methylation patterns (common in DNA from other organisms like eukaryotes), which vastly improves cloning efficiency. supE44 / glnV44 suppressor mutation: This helps ensure that the entire protein sequence encoded by a plasmid gene is translated correctly, by reading through certain premature stop codons. thi-1: This indicates a nutritional requirement for thiamine (Vitamin B1) in the growth medium. λ⁻ (lambda-minus): The strain lacks the lambda prophage. Culture and Handling Culture Medium: Standard Luria–Bertani (LB) broth or agar plates are the standard for cultivation. Growth Conditions: Cultures are grown aerobically. Temperature: Incubation is typically performed at 37°C. Storage: The strain can be maintained on agar slants at +4°C for 1-2 weeks. For long-term preservation, it should be frozen in a glycerol stock solution and stored at -80°C. BioVector® MM294 大肠杆菌 (E. coli) 菌株是一种广泛使用的实验室菌株，源自 K-12 谱系。它主要用于克隆和质粒 DNA 的生产，因为其具有较高的外源 DNA 摄取效率（转化能力）和在质粒小量制备过程中产生高产量纯净质粒 DNA 的能力。 描述和特征 生物体： Escherichia coli (大肠杆菌)。 菌株名称： K12 MM294，或简称 MM294。 来源： 源自 Matthew Meselson 的实验室。 主要用途： 在分子生物学工作流程中，用作质粒转化和增殖的稳定宿主。 表型： 具有标准的野生型外观。 质粒状态： 该菌株不含任何内源质粒。 生物安全级别： 通常归类为生物安全 1 级 (BSL-1)。 关键遗传特征（基因型） MM294 的特定基因修饰提供了克隆的功能优势： F⁻： 这意味着该菌株在细菌接合过程中是受体，因此不能通过接合作用充当遗传物质的供体。 endA1 突变： 该突变使细胞内部的核酸内切酶 I 失活。这种酶的缺失至关重要，因为它可以防止在纯化过程中质粒 DNA 被降解，从而提高产量和 DNA 纯度。 hsdR17 (rK⁻mK⁺) 突变： 这会影响限制-修饰系统。该菌株不能切割（限制）缺乏特定甲基化模式的外源 DNA（在来自真核生物等其他生物体的 DNA 中很常见），这极大地提高了克隆效率。 supE44 / glnV44 抑制突变： 这有助于确保质粒基因编码的整个蛋白质序列被正确翻译，通过读取某些提前终止密码子来实现。 thi-1： 这表明在生长培养基中需要硫胺素（维生素 B1）作为营养补充。 λ⁻（lambda 阴性）： 该菌株不含 lambda 原噬菌体。 培养和处理 培养基： 标准的 Luria–Bertani (LB) 肉汤或琼脂平板是培养的标准培养基。 生长条件： 培养物需在有氧条件下生长。 温度： 通常在 37°C 下孵育。 储存： 菌株可以在 +4°C 的琼脂斜面上维持 1-2 周。为了长期保存，应冷冻在甘油储备液中，并储存在 -80°C。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net

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