GIST-48贴壁人胃肠道间质瘤 (GIST) 细胞系NTCC® BioVector NTCC保藏中心
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BioVector® NTCC® GIST-48贴壁人胃肠道间质瘤 (GIST) 细胞系NTCC®
The GIST-48 cell line is an adherent human gastrointestinal stromal tumor (GIST) cell line established from a tumor that progressed during imatinib therapy. It is a key model for studying drug resistance due to its specific combination of KIT gene mutations. Key Characteristics Organism: Homo sapiens (Human) Disease: Gastrointestinal Stromal Tumor (GIST), specifically imatinib-resistant. Growth Properties: Adherent monolayer. Morphology: Spindle-shaped/epithelial-like cells. Key Mutations: The line is characterized by two KIT mutations: Primary Mutation: Homozygous KIT exon 11 V560D point mutation. Secondary (Resistance) Mutation: Heterozygous KIT exon 17 D820A missense mutation. Immunomarker: Expresses c-kit (CD117) and CD34. Derived Subline: The GIST48B subline was derived from GIST-48 after drug pressure, resulting in nearly undetectable KIT expression. Culture Conditions Culture Medium: F10 medium supplemented with 15% Fetal Bovine Serum (FBS), serum extender, and bovine pituitary extract. Other protocols use a 50:50 mixture of Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) with 15% FBS. Growth Temperature: 37°C. CO₂ Concentration: 5% CO₂ in a humidified incubator. Subculture Routine: GIST-48 cells are typically incubated for 120 hours in experiments, suggesting a slow growth rate similar to other GIST lines. They are often split 1:3 when subculturing. GIST-48 细胞系是一种贴壁人胃肠道间质瘤 (GIST) 细胞系，建立自一名在伊马替尼治疗期间出现疾病进展的患者肿瘤。由于其 KIT 基因突变的特定组合，它是研究耐药性的重要模型。 主要特征 生物体： Homo sapiens (人) 疾病： 胃肠道间质瘤 (GIST)，具体表现为伊马替尼耐药性。 生长特性： 贴壁单层生长。 形态： 纺锤形/上皮样细胞。 关键突变： 该细胞系具有两个 KIT 突变： 原发突变： KIT 外显子 11 纯合 V560D 点突变。 继发（耐药）突变： KIT 外显子 17 杂合 D820A 错义突变。 免疫标记： 表达 c-kit (CD117) 和 CD34。 衍生亚系： GIST48B 亚系是在药物压力下从 GIST-48 衍生而来，导致几乎检测不到 KIT 表达。 培养条件 培养基： F10 培养基，补充 15% 胎牛血清 (FBS)、血清延伸物和牛垂体提取物。其他方案使用 Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) 的 50:50 混合物和 15% FBS。 生长温度： 37°C。 CO₂ 浓度： 在 37°C、5% CO₂ 的恒温培养箱中培养。 传代程序： GIST-48 细胞在实验中通常培养 120 小时，表明其生长速度较慢，类似于其他 GIST 细胞系。传代时通常按 1:3 的比例稀释。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
The GIST-48 cell line is an adherent human gastrointestinal stromal tumor (GIST) cell line established from a tumor that progressed during imatinib therapy. It is a key model for studying drug resistance due to its specific combination of KIT gene mutations. Key Characteristics Organism: Homo sapiens (Human) Disease: Gastrointestinal Stromal Tumor (GIST), specifically imatinib-resistant. Growth Properties: Adherent monolayer. Morphology: Spindle-shaped/epithelial-like cells. Key Mutations: The line is characterized by two KIT mutations: Primary Mutation: Homozygous KIT exon 11 V560D point mutation. Secondary (Resistance) Mutation: Heterozygous KIT exon 17 D820A missense mutation. Immunomarker: Expresses c-kit (CD117) and CD34. Derived Subline: The GIST48B subline was derived from GIST-48 after drug pressure, resulting in nearly undetectable KIT expression. Culture Conditions Culture Medium: F10 medium supplemented with 15% Fetal Bovine Serum (FBS), serum extender, and bovine pituitary extract. Other protocols use a 50:50 mixture of Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) with 15% FBS. Growth Temperature: 37°C. CO₂ Concentration: 5% CO₂ in a humidified incubator. Subculture Routine: GIST-48 cells are typically incubated for 120 hours in experiments, suggesting a slow growth rate similar to other GIST lines. They are often split 1:3 when subculturing. GIST-48 细胞系是一种贴壁人胃肠道间质瘤 (GIST) 细胞系，建立自一名在伊马替尼治疗期间出现疾病进展的患者肿瘤。由于其 KIT 基因突变的特定组合，它是研究耐药性的重要模型。 主要特征 生物体： Homo sapiens (人) 疾病： 胃肠道间质瘤 (GIST)，具体表现为伊马替尼耐药性。 生长特性： 贴壁单层生长。 形态： 纺锤形/上皮样细胞。 关键突变： 该细胞系具有两个 KIT 突变： 原发突变： KIT 外显子 11 纯合 V560D 点突变。 继发（耐药）突变： KIT 外显子 17 杂合 D820A 错义突变。 免疫标记： 表达 c-kit (CD117) 和 CD34。 衍生亚系： GIST48B 亚系是在药物压力下从 GIST-48 衍生而来，导致几乎检测不到 KIT 表达。 培养条件 培养基： F10 培养基，补充 15% 胎牛血清 (FBS)、血清延伸物和牛垂体提取物。其他方案使用 Dulbecco's Modified Eagle's Medium/Ham's F-12 (DMEM/F-12) 的 50:50 混合物和 15% FBS。 生长温度： 37°C。 CO₂ 浓度： 在 37°C、5% CO₂ 的恒温培养箱中培养。 传代程序： GIST-48 细胞在实验中通常培养 120 小时，表明其生长速度较慢，类似于其他 GIST 细胞系。传代时通常按 1:3 的比例稀释。 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心 电话:400-800-2947 工作QQ/微信同号:1843439339 网址 www.biovector.net
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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