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pComb3XSS噬菌体展示技术表达载体BioVector® Filamentous phage display plasmid BioVector NTCC保藏中心

  • 价  格:¥49950
  • 货  号:BioVector®-pComb3XSS
  • 产  地:北京
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BioVector® NTCC® pComb3XSS噬菌体展示技术表达载体BioVector® Filamentous phage display plasmid

基本信息

质粒名称: pComb3XSS BioVector®

质粒类型: 表达载体,噬菌体展示系统

宿主: 大肠杆菌(E. coli)

主要用途

用于 噬菌体展示技术(Phage Display)

蛋白质-肽库构建

蛋白质与抗体相互作用研究

高通量筛选结合位点

质粒特性

复制起点(Ori): ColE1

选择标记: 抗生素抗性(通常为氨苄青霉素)

融合标签: 可构建噬菌体展示融合蛋白(通常为 pIII 蛋白)

多克隆位点 (MCS): 允许插入目标基因序列

表达信息

适合在 E. coli 中表达融合蛋白

可用于噬菌体展示文库构建

注意事项

遵守生物安全规范(通常 BSL-1)

储存条件:-20℃ 长期保存

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net

General Information

Name: pComb3XSS BioVector®

Type: Filamentous phage display plasmid (phagemid)

Host Range: E. coli (commonly used strains compatible with phage display)

Primary Use:

Antibody library construction

Phage display of antibody fragments (scFv, Fab)

Screening and affinity selection of binding proteins

Backbone Features

Origin of Replication:

E. coli origin for high-copy plasmid maintenance

Filamentous phage origin for packaging into phage particles (requires helper phage)

Selectable Marker:

Antibiotic resistance gene for selection in E. coli (depends on vendor/source)

Multiple Cloning Site (MCS):

Supports insertion of antibody variable regions or other display constructs

Includes restriction sites designed for library construction (no sequences provided)

Display Region:

Gene fusion region enabling display on phage coat protein (commonly pIII)

Allows surface presentation of inserted antibody fragments

Stop/Start Configuration:

“SS” in pComb3XSS refers to an improved stop/start configuration enhancing expression and display efficiency

Expression / Display Characteristics

Supports expression of antibody fragments in a format suitable for phage pIII fusion

Compatible with helper phage systems for packaging

Designed for improved phage titers and display efficiency compared to earlier pComb vectors

Typical Applications

Construction of synthetic, immune, or semi-synthetic antibody libraries

Biopanning (phage selection)

Discovery of binders to proteins, peptides, glycans, small molecules, or cells

Research in immunology, structural biology, and directed evolution

Storage

Typically stored as purified plasmid DNA or as glycerol stocks

Maintain under standard DNA storage conditions (details vary by provider)

Biosafety Information

BSL-1 organism/plasmid for routine laboratory handling

Non-pathogenic phagemid requiring helper phage for packaging

Standard laboratory safety practices recommended
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net


Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net

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