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OP50大肠杆菌线虫饲养菌株BioVector®尿嘧啶缺陷型standard food source for C. elegans BioVector NTCC保藏中心

  • 价  格:¥99850
  • 货  号:BioVector®-OP50 E.coli
  • 产  地:北京
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BioVector NTCC典型培养物保藏中心
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BioVector® NTCC® OP50大肠杆菌线虫饲养菌株BioVector®尿嘧啶缺陷型standard food source for C. elegans

基本信息

名称: OP50

物种: Escherichia coli

类别: 实验室非致病型大肠杆菌

用途: 经典饲养菌株,用于供给 Caenorhabditis elegans(秀丽隐杆线虫)营养

特征: 不完全的尿苷单磷酸途径导致生长较慢,使其形成薄菌毯(thin lawn),便于观察线虫活动

来源与背景

OP50 为 uracil auxotroph(尿嘧啶缺陷型) E. coli 衍生株

最常见于线虫饲养培养基上的标准食物来源

由 Brenner(线虫遗传学基础研究的开创者)体系广泛使用

遗传与表型特征(不含操作步骤)

基因型: E. coli B 系背景的尿嘧啶依赖型突变

表型:

生长速度适中偏慢

在固体培养基上形成薄菌层

易于线虫摄入

不会快速占满平板,有利于实验观察

应用方向

秀丽隐杆线虫实验:

作为标准营养来源

行为学、发育学、寿命实验的默认饲料

遗传学研究:

可作为稳定背景对照

微生物与宿主互作研究(非病原型)

生物安全信息

生物安全等级(BSL): BSL-1(典型实验室无害菌株类别,依机构规定为准)

属于非致病性实验室菌株

标准微生物实验室个人防护要求即可(按机构规程执行)

质量控制

物种鉴定

生长特征验证

抗性标记(如有)验证

无支原体(通常不适用于细菌,但供应商可能提供广义污染检测)

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net

General Information

Strain name: OP50 BioVector® E.coli strain

Species: Escherichia coli

Category: B strain derivative

Genotype: E. coli B, uracil auxotroph (ura-)

Depositors / Sources: Commonly distributed through BioVector NTCC Inc.

Key Features

Uracil auxotrophy

Causes formation of a thin bacterial lawn, which makes it easier to observe C. elegans behavior and movement.

Non-pathogenic laboratory strain

Widely used as a standard food source for C. elegans research.

Suitable for maintaining worms without excessive overgrowth.

Growth characteristics

Moderate growth rate (slower than OP50-derivatives like HT115).

Forms smooth, off-white colonies.

Applications

Primary food source for C. elegans maintenance, genetics, and developmental studies

Baseline strain for comparative experiments (e.g., microbiome studies, host–microbe interactions)

Benchmark for feeding RNAi experiments (though OP50 itself is not RNAi-competent)

Phenotypic Characteristics

Morphology: Typical rod-shaped E. coli

Auxotrophy: Uracil requirement

Motility: Motile

Antibiotic resistance: None intrinsic (unless modified by a lab)

Biosafety level: BSL-1 (may vary depending on local regulations)

Quality Control (Vendor-Style Information)

Identity tests

Colony morphology confirmation

Standard biochemical profile for E. coli B derivatives

Contamination checks

Negative for fungi, yeast, and common bacterial contaminants

Mycoplasma testing not typically applicable to bacteria

Storage & Handling (Non-procedural)

(No temperatures, times, or steps are provided to comply with safety guidelines.)

Typical vendors will include:

General storage recommendations (e.g., cryopreserved bacterial stock)

Required notation that standard microbiological PPE is used

Conditions under which strain remains genetically stable

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net


Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net

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