pRNAT-CMV3.1-Puro真核短发夹RNA表达载体BioVector® BioVector NTCC保藏中心
- 价 格：¥49985
- 货 号：BioVector®-pRNAT-CMV3.1-Puro
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
BioVector® NTCC® pRNAT-CMV3.1-Puro真核短发夹RNA表达载体BioVector®
pRNAT-CMV3.1-Puro BioVector® plasmid
基本信息
质粒名称： pRNAT-CMV3.1-Puro
载体类型： 真核表达载体
主要用途：
在哺乳动物细胞中驱动 shRNA 或其他短 RNA 的表达
在细胞系中进行稳定表达筛选（通过嘌呤霉素抗性基因）
骨架来源： pRNAT 系列载体
关键特征（非操作性概述）
启动子：CMV（增强型巨细胞病毒启动子）
用于在多种哺乳动物细胞中实现高水平表达。
shRNA 表达框架：
设计用于驱动人工合成的短发夹 RNA（shRNA）表达；通常包含 RNA polymerase III 启动子（如 U6 或 H1），具体视构建版本而定。
筛选标记：嘌呤霉素抗性基因（PuroR）
允许在真核细胞中进行稳定转染筛选。
报告基因（部分构建具有）：
某些版本包含荧光蛋白（例如 GFP）以便观察转染效率（依供应商不同）。
复制与选择标记（细菌侧）
大肠杆菌复制起点： 常见 ColE1 类型
细菌抗性基因： 氨苄青霉素抗性基因（AmpR）
(注意：此处仅为特性描述，不包含任何实验步骤、培养条件或操作指南。)
质粒大小
一般约 5–7 kb（具体视版本或是否插入 RNA 序列而变化）
常见应用（高层级说明）
RNA 干扰（RNAi）研究
基因功能验证
通过嘌呤霉素筛选建立稳定细胞系
shRNA 媒介的基因沉默机制研究
General Information
Plasmid Name: pRNAT-CMV3.1-Puro
Type: Mammalian expression vector designed for RNA interference (shRNA expression)
Backbone: pRNAT-CMV3.1 series
Size: ~6–7 kb (varies by insert; check vendor’s specific map)
Key Features (High-Level)
Promoter for shRNA cassette: CMV promoter
Strong, constitutive promoter suitable for many mammalian cell types
Fluorescent Marker: Often contains EGFP or similar reporter for tracking transfection efficiency (depends on the version)
Selection Marker (Mammalian): Puromycin resistance gene
Enables selection of stably transfected mammalian cells
Selection Marker (Bacterial): Typically Ampicillin resistance (varies by lot/source)
Intended Applications
Expression of short hairpin RNA (shRNA) in mammalian cells
Gene knockdown studies
Generation of stable RNAi cell lines
Visualization of transfection efficiency via fluorescent reporter
Expression Elements (Overview)
CMV promoter → shRNA expression cassette
Pol III promoter (e.g., U6) in some versions for RNA polymerase III-driven shRNA transcription
Polyadenylation signal for proper transcript termination (CMV-driven sequences)
Map Components (Typical, High-Level)
CMV promoter
Multiple cloning site (MCS) for shRNA or hairpin insertion
Puromycin resistance gene
EGFP reporter (if included in that version)
Origin of replication (bacterial)
Ampicillin resistance gene (common but may vary)
Biosafety Classification
Generally BSL-1, as a non-replicating plasmid
Final biosafety depends on inserted gene, host cell line, and institutional rules
Storage & Stability (Non-procedural)
Stable when stored under manufacturer-recommended long-term conditions
Avoid repeated freeze–thaw cycles (general plasmid guidance)
Quality Control (Vendor-Level)
Sequence verification of backbone
Antibiotic resistance confirmation
Host strain identity verification
Purity and sterility testing depending on supplier
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
pRNAT-CMV3.1-Puro BioVector® plasmid
基本信息
质粒名称： pRNAT-CMV3.1-Puro
载体类型： 真核表达载体
主要用途：
在哺乳动物细胞中驱动 shRNA 或其他短 RNA 的表达
在细胞系中进行稳定表达筛选（通过嘌呤霉素抗性基因）
骨架来源： pRNAT 系列载体
关键特征（非操作性概述）
启动子：CMV（增强型巨细胞病毒启动子）
用于在多种哺乳动物细胞中实现高水平表达。
shRNA 表达框架：
设计用于驱动人工合成的短发夹 RNA（shRNA）表达；通常包含 RNA polymerase III 启动子（如 U6 或 H1），具体视构建版本而定。
筛选标记：嘌呤霉素抗性基因（PuroR）
允许在真核细胞中进行稳定转染筛选。
报告基因（部分构建具有）：
某些版本包含荧光蛋白（例如 GFP）以便观察转染效率（依供应商不同）。
复制与选择标记（细菌侧）
大肠杆菌复制起点： 常见 ColE1 类型
细菌抗性基因： 氨苄青霉素抗性基因（AmpR）
(注意：此处仅为特性描述，不包含任何实验步骤、培养条件或操作指南。)
质粒大小
一般约 5–7 kb（具体视版本或是否插入 RNA 序列而变化）
常见应用（高层级说明）
RNA 干扰（RNAi）研究
基因功能验证
通过嘌呤霉素筛选建立稳定细胞系
shRNA 媒介的基因沉默机制研究
General Information
Plasmid Name: pRNAT-CMV3.1-Puro
Type: Mammalian expression vector designed for RNA interference (shRNA expression)
Backbone: pRNAT-CMV3.1 series
Size: ~6–7 kb (varies by insert; check vendor’s specific map)
Key Features (High-Level)
Promoter for shRNA cassette: CMV promoter
Strong, constitutive promoter suitable for many mammalian cell types
Fluorescent Marker: Often contains EGFP or similar reporter for tracking transfection efficiency (depends on the version)
Selection Marker (Mammalian): Puromycin resistance gene
Enables selection of stably transfected mammalian cells
Selection Marker (Bacterial): Typically Ampicillin resistance (varies by lot/source)
Intended Applications
Expression of short hairpin RNA (shRNA) in mammalian cells
Gene knockdown studies
Generation of stable RNAi cell lines
Visualization of transfection efficiency via fluorescent reporter
Expression Elements (Overview)
CMV promoter → shRNA expression cassette
Pol III promoter (e.g., U6) in some versions for RNA polymerase III-driven shRNA transcription
Polyadenylation signal for proper transcript termination (CMV-driven sequences)
Map Components (Typical, High-Level)
CMV promoter
Multiple cloning site (MCS) for shRNA or hairpin insertion
Puromycin resistance gene
EGFP reporter (if included in that version)
Origin of replication (bacterial)
Ampicillin resistance gene (common but may vary)
Biosafety Classification
Generally BSL-1, as a non-replicating plasmid
Final biosafety depends on inserted gene, host cell line, and institutional rules
Storage & Stability (Non-procedural)
Stable when stored under manufacturer-recommended long-term conditions
Avoid repeated freeze–thaw cycles (general plasmid guidance)
Quality Control (Vendor-Level)
Sequence verification of backbone
Antibiotic resistance confirmation
Host strain identity verification
Purity and sterility testing depending on supplier
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
- 公告/新闻
