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Vibrio parahaemolyticus NTCC®副溶血弧菌 BioVector NTCC保藏中心

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  • 货  号:BioVector®-Vibrio parahaemolyticus
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BioVector® NTCC® Vibrio parahaemolyticus NTCC®副溶血弧菌

基本信息

学名: Vibrio parahaemolyticus NTCC®

中文名称: 副溶血弧菌

分类:

纲:Gammaproteobacteria

科:Vibrionaceae

形态:

弧形或杆状革兰氏阴性细菌

具极鞭毛,可运动

自然宿主 / 来源:

广泛存在于温暖海水

常与海产品(贝类、甲壳类)相关

重要特征
生理特性(高层次,不含可操作条件)

耐盐性强,典型海洋弧菌特征

在温暖环境中生长较快

能发酵多种碳源

具有溶血能力(Kanagawa 现象因菌株而异)

致病性相关(高层描述)

可引起 急性胃肠炎,常因食用未熟海产品

关键毒力因子(因菌株而异):

TDH(thermostable direct hemolysin)

TRH(TDH-related hemolysin)

T3SS1/T3SS2 分泌系统

遗传学信息

染色体数量: 双染色体结构(典型弧菌特征)

常用参考基因组:

V. parahaemolyticus RIMD 2210633(全球研究标准参考株)

分子鉴定标志物(高层次):

toxR(物种鉴定)

tdh、trh(毒力基因检测)

应用场景

食品安全与公共卫生研究

海洋微生物生态研究

病原检测技术开发

毒力基因与基因调控研究

水产养殖疾病监测

生物安全信息

生物安全等级(一般): BSL-2(因其致病性,对人类有感染风险)

潜在风险:

引起胃肠炎

海水或海产品处理行业的职业暴露

实验室注意事项(仅原则性叙述):

适当防护

避免气溶胶

按法规处置废弃物

(不提供任何操作步骤、培养条件或生长参数。)

常见研究用菌株列表(不含获取方式)

RIMD 2210633(日本临床分离株,研究使用最广)

ATCC 17802(标准参考株)

ATCC 27519(另一常用参考株)

临床/海水/海产品环境分离株(毒力基因型差异显著)

1. General Information

Species: Vibrio parahaemolyticus

Taxonomy:

Phylum: Proteobacteria

Class: Gammaproteobacteria

Order: Vibrionales

Family: Vibrionaceae

Gram Stain: Gram-negative

Morphology: Curved or straight rods, motile by polar flagella

Natural Habitat: Marine and estuarine environments (saltwater)

Disease Association: A leading cause of seafood-associated gastroenteritis in humans

2. Key Characteristics
Biological Features

Halophilic (salt-requiring)

Facultatively anaerobic

Produces thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) in pathogenic strains

Shows typical “Vibrio” motility patterns

Differentiating Traits

Oxidase-positive

Ferments glucose

Growth favored by moderate salinity
(Details on conditions omitted for safety.)

3. Common Research Uses

Studies of marine pathogenic bacteria

Seafood safety and contamination monitoring

Virulence factor research (e.g., TDH, TRH toxins)

Genomics, epidemiology, and population structure studies

Host–pathogen interaction models

Environmental stress response studies (e.g., salinity, temperature)

4. Biosafety Information

Biosafety Level: BSL-2 (varies by jurisdiction; consult local regulations)

Associated Risks: Gastroenteritis if ingested; wound infections possible through exposure to contaminated seawater

Transmission Routes:

Contaminated raw or undercooked seafood

Open wound exposure to marine water containing the bacteria

Recommended Precautions:

Standard BSL-2 PPE

Avoid aerosol generation

Follow institutional biosafety guidance

(No procedural details provided.)

5. Strain Variability

V. parahaemolyticus includes numerous strains and serotypes, commonly characterized by:

Serogroups: O1–O12; K antigen variations

Pandemic clone: O3:K6 and related serovariants (notable for widespread outbreaks)

Genomic markers:

tdh, trh virulence genes

Type III secretion systems (T3SS1, T3SS2)

Different strains may vary in virulence, habitat range, and antibiotic susceptibility.

6. Genomic Information

Genome Size: ~5.0–5.3 Mb

Genome Structure: Two circular chromosomes (typical of Vibrio species)

GC Content: ~45%

Key Genomic Features:

Toxin genes (tdh, trh)

Environmental stress response pathways

Motility and chemotaxis systems

Type III secretion system gene clusters

7. Applications in Industry and Environment

Monitoring marine water quality

Seafood industry pathogen surveillance

Tracking environmental spread linked to warming oceans

Genetic studies on adaptation to salinity and temperature shifts
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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