PGs2 CRISPR Activation Plasmid (h2) BioVector NTCC保藏中心

BioVector® NTCC® PGs2 CRISPR Activation Plasmid (h2)

PGs2 CRISPR Plasmids
BioVector NTCC Inc.

Target species: human
CRISPR/Cas9 KO Plasmids consists of PGs2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
Gene knockdown or activation can be assayed using PGs2 Antibody (D-9): sc-514306
All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells

Gene Info
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession Number Protein Accession Number OMIM™ Number
Human TPGS2 Entrez Gene ID: 25941 18q12.2 XM_005258242, XM_011525917, XM_017025699, XM_017025700, XM_017025701, XM_017025702, XM_017025703, NM_001271949, NM_001271950, NM_001271951, NM_001271952, NM_001271953, NM_001271954, NM_001271955, NM_001271956, NM_001330572, NM_015476 UniProt ID: Q68CL5 n/a

Description

20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
PGs2 CRISPR/Cas9 Knockout (KO) Plasmid (h) consists of a pool of three plasmids each encoding the Cas9 nuclease and a PGs2-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency
gRNA sequences are derived from the GeCKO (v2) library and direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
For selection of cells containing a successful Cas9-induced DSB, co-transfection with PGs2 HDR Plasmid (h) (sc-411842-HDR) is recommended
Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PGs2 Antibody (D-9): sc-514306

HDR Plasmid


Double Nickase Plasmids


CRISPR Activation Plasmids


Lentiviral Activation Particles

Description

20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
PGs2 HDR Plasmid (h) is recommended for co-transfection with PGs2 CRISPR/Cas9 KO Plasmid (h)
PGs2 HDR Plasmid (h) consists of a pool of 2-3 plasmids, each containing a homology-directed DNA repair (HDR) template corresponding to the cut sites generated by the PGs2 CRISPR/Cas9 KO Plasmid (h)
Each HDR plasmid contains two 800 bp homology arms designed to specifically bind to the genomic DNA surrounding the corresponding Cas9-induced double strand DNA break site
Each HDR Plasmid inserts a puromycin resistance gene to enable selection of stable knockout (KO) cells and an RFP (Red Fluorescent Protein) gene to visually verify transfection
The puromycin resistance and RFP genes are flanked by two LoxP sites to allow for further processing by the Cre Vector sc-418923



Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net