pBIB-BASTA-GFP植物双元表达质粒BioVector®载体 BioVector NTCC保藏中心
- 价 格：¥49950
- 货 号：BioVector®-pBIB-BASTA-GFP
- 产 地：北京
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- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
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地址：北京
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BioVector® NTCC® pBIB-BASTA-GFP植物双元表达质粒BioVector®载体
The promoter of PzIPT1 was inserted into a binary vector pBIB-BASTA-GUS modified from pBIB vector (Becker, 1990) at the HindIII and SalI sites to make pPzIPT1::GUS. The PzIPT1 promoter was also recombined into the Gateway-compatible pFYTAG binary vector to drive the expression of fused coding regions of histone 2A (HTA6; At5g59870) and enhanced YFP (EYFP; Zhang et al., 2005). The binary vector pBIB-BASTA-GFP (Ge et al., 2011) was modified to a Gateway-compatible destination vector, pBIB-BASTA-GFP-GWR, by inserting the Gateway module at HindIII and XbaI sites for promoter analyses.
Deletion fragments were amplified by PCR from cloned PzIPT1 promoter and transferred into pDONR/Zeo vector by Gateway in vitro DNA recombination for sequencing analysis. Following sequence verification, these truncated promoter fragments were in vitro recombined into pBIB-BASTA-GFP-GWR and pBIB-BASTA-GUS-GWR (Yuan et al., 2007) to create final binary transformation constructs. IPTPromPB2 was used as a reverse primer for all ten 5′-deletions. Forward primers (∆761PB1, ∆531PB1, ∆368PB1, ∆255PB1, ∆154PB1, ∆130PB1, ∆105PB1, ∆87PB1, ∆63PB1, ∆39PB1) were designed according to the positions of the deletions in the PzIPT1 promoter. The deletion constructs ∆88-95, ∆64-87, and ∆40-63 were created according to the manual of QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) using primers ∆88-95F, ∆88-95R, ∆64-87F, ∆64-87R, ∆40-63F, and ∆40-63R.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
The promoter of PzIPT1 was inserted into a binary vector pBIB-BASTA-GUS modified from pBIB vector (Becker, 1990) at the HindIII and SalI sites to make pPzIPT1::GUS. The PzIPT1 promoter was also recombined into the Gateway-compatible pFYTAG binary vector to drive the expression of fused coding regions of histone 2A (HTA6; At5g59870) and enhanced YFP (EYFP; Zhang et al., 2005). The binary vector pBIB-BASTA-GFP (Ge et al., 2011) was modified to a Gateway-compatible destination vector, pBIB-BASTA-GFP-GWR, by inserting the Gateway module at HindIII and XbaI sites for promoter analyses.
Deletion fragments were amplified by PCR from cloned PzIPT1 promoter and transferred into pDONR/Zeo vector by Gateway in vitro DNA recombination for sequencing analysis. Following sequence verification, these truncated promoter fragments were in vitro recombined into pBIB-BASTA-GFP-GWR and pBIB-BASTA-GUS-GWR (Yuan et al., 2007) to create final binary transformation constructs. IPTPromPB2 was used as a reverse primer for all ten 5′-deletions. Forward primers (∆761PB1, ∆531PB1, ∆368PB1, ∆255PB1, ∆154PB1, ∆130PB1, ∆105PB1, ∆87PB1, ∆63PB1, ∆39PB1) were designed according to the positions of the deletions in the PzIPT1 promoter. The deletion constructs ∆88-95, ∆64-87, and ∆40-63 were created according to the manual of QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) using primers ∆88-95F, ∆88-95R, ∆64-87F, ∆64-87R, ∆40-63F, and ∆40-63R.
Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net
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