A19 E.coli strain BioVector®大肠杆菌菌株 BioVector NTCC保藏中心

BioVector® NTCC® A19 E.coli strain BioVector®大肠杆菌菌株

特性 详情
生物体 大肠杆菌 K-12 (Migula) Castellani and Chalmers
菌株名称 A19 NTCC®
形态 革兰氏阴性、杆状细菌
生长方式 肉汤或琼脂培养（好氧条件）
核型 Hfr（高频重组）
基因型注释 his 标记（可能回复）；酵母提取物可满足的未识别生长要求；内切核酸酶 I (endA) 和 RecBCD 缺陷
生物安全等级 BSL 1 级（非致病性实验室菌株）
登录号 CGSC #7376；BGSC ECE 19
培养条件
培养基：
推荐：Luria-Bertani (LB) 液体培养基或 LB 琼脂平板。在特定实验方案中也会使用 2x YT 或 Tryptic Soy Broth (TSB) 等其他培养基。
最佳温度：37°C。
气氛：好氧条件。
生长速度：快速；在有利条件下，繁殖时间短至 20 分钟。
应用
A19 菌株是几个研究领域的基础工具：
无细胞蛋白合成 (CFPS)：主要应用是生产 A19 细胞的 S30 提取物。这些提取物用于体外转录和翻译系统，在没有完整活细胞的情况下生产蛋白质，这对于合成膜蛋白或难以在体内表达的蛋白质特别有用。
遗传学研究：由于其 Hfr 状态和充分了解的基因组，它被用作研究细菌遗传学、基因调控和 DNA 修复机制的模型生物。
生物技术/蛋白质生产：作为重组 DNA 技术和酶及其他蛋白质高产生产的标准宿主。
诱变研究：已用于分离对某些抗生素（如链佐丝菌素）具有抗性的突变体的研究，以了解耐药机制。
General Information
Property Detail
Organism Escherichia coli K-12 (Migula) Castellani and Chalmers
Strain Designation BioVector® A19
Morphology Gram-negative, rod-shaped bacterium
Growth Mode Broth or agar culture (aerobic conditions)
Karyotype Hfr (High Frequency of Recombination)
Genotype Notes his marker (may revert); an unidentified growth requirement satisfied by yeast extract; deficient in Endonuclease I (endA) and RecBCD
Biosafety Level BSL 1 (Non-pathogenic laboratory strain)
Accession Numbers CGSC #7376; BGSC ECE 19
Culture Conditions
Culture Medium:
Recommended: Luria-Bertani (LB) liquid medium or LB agar plates. Other media like 2x YT or Tryptic Soy Broth (TSB) are also used in specific protocols.
Optimal Temperature: 37°C.
Atmosphere: Aerobic conditions.
Growth Rate: Rapid; takes as little as 20 minutes to reproduce under favorable conditions.
Applications
The A19 strain is a foundational tool in several research areas:
Cell-Free Protein Synthesis (CFPS): A major application is the production of S30 extracts from A19 cells. These extracts are used in in vitro transcription and translation systems to produce proteins without intact living cells, which is particularly useful for synthesizing membrane proteins or proteins that are difficult to express in vivo.
Genetics Research: Due to its Hfr status and well-understood genome, it is used as a model organism to study bacterial genetics, gene regulation, and DNA repair mechanisms.
Biotechnology/Protein Production: Serves as a standard host for recombinant DNA technology and the high-yield production of enzymes and other proteins.
Mutagenesis Studies: Has been used in studies to isolate mutants with resistance to certain antibiotics like streptothricin to understand drug resistance mechanisms.

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net