KGN NTCC®永生化人卵巢颗粒样瘤细胞株immortalized human ovarian granulosa-like tumor cell line

价　　格：¥99860
货　　号：NTCC®-KGN CELL
产　　地：北京

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NTCC® KGN cell line is an immortalized human ovarian granulosa-like tumor cell line widely used as an in vitro model for studying ovarian function, hormone regulation, and diseases like polycystic ovary syndrome (PCOS) and ovarian cancer.

KGN Cells

Key Characteristics

Origin: Derived from a stage III ovarian granulosa cell tumor from a 63-year-old Japanese woman.
Morphology and Growth: Adherent cells with a fibroblast-like morphology and a relatively slow population doubling time of around 46.4 hours.
Functionality: KGN cells retain key physiological characteristics of normal granulosa cells, including the expression of functional follicle-stimulating hormone (FSH) receptors and steroidogenic activity. They can produce progesterone and estrogen, particularly after stimulation with agents like forskolin or dibutyryl cyclic adenosine monophosphate (dbcAMP).
Genetic Profile: The cell line has an abnormal karyotype (45, XX, 7q-, -22) and features a common somatic mutation in the FOXL2 gene (p.Cys134Trp, or c.402C>G), which is found in almost all adult-type granulosa cell tumors.
Biosafety Level: Biosafety Level 1.

Research Applications

Due to their unique properties, KGN cells serve as a valuable research tool for:

Investigating the molecular mechanisms of ovarian follicle development and function.
Studying hormonal signaling pathways, especially those related to FSH and steroidogenesis.
Modeling reproductive and endocrine disorders, such as PCOS.
Exploring potential therapeutic targets for ovarian granulosa cell tumors and testing anti-cancer drugs.

Researchers should be aware that KGN cells have some unique features compared to primary granulosa cells, such as extremely high expression of the antioxidant gene TXNRD1, which might affect studies focused on the interplay between steroidogenesis, reactive oxygen species, and antioxidants.

NTCC® KGN细胞系是一种永生化人卵巢颗粒样瘤细胞系，被广泛用作研究卵巢功能、激素调节以及多囊卵巢综合征（PCOS）和卵巢癌等疾病的体外模型。

主要特点

起源：源自一名63岁日本女性的III期卵巢颗粒细胞瘤。
形态和生长：贴壁细胞，具有成纤维细胞样形态，倍增时间相对较慢，约为46.4小时。
功能性： KGN细胞保留了正常颗粒细胞的关键生理特征，包括表达功能性促卵泡激素（FSH）受体和类固醇生成活性。它们可以产生孕酮和雌激素，特别是在用福司可林（forskolin）或二丁酰环磷酸腺苷（dbcAMP）等试剂刺激后。
基因概况：该细胞系具有异常核型（45, XX, 7q-, -22），并具有FOXL2基因中常见的体细胞突变（p.Cys134Trp，或c.402C>G），该突变几乎存在于所有成人型颗粒细胞瘤中。
生物安全级别：生物安全一级。