HEK293::TMEM192-3xHA FIP200-/- ::mNEON-YIPF4,HEK293 FIP200-/-细胞BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Cell line name: HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HEK293::TMEM192-3xHA FIP200-/- ::mNEON-YIPF4; HEK293 FIP200-/-
Cross-references: Wikidata; Q127381649
References identifiers: PubMed=37757899;
Comments: Characteristics: Using CRISPR/Cas9 TMEM192 was C-terminally tagged on both alleles with a 3xHA epitope (from parent cell line).
Comments: Characteristics: Using CRISPR/Cas9 YIPF4 was N-terminally tagged on both alleles with mNeonGreen (PubMed=37757899).
Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:15574; RB1CC1.
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
Comments: Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Comments: Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_D1KQ ! HEK293::TMEM192-3xHA RB1CC1-/-
Sex of cell: Female
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC® CVCL_D1JA
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培养方法：
Medium培养基：NTCC® Cell line name: HEK293::TMEM192-3xHA RB1CC1-/- mNEON-YIPF4 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

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