CHO.SIINFEKL/H-2Kb细胞BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® CHO-SIINFEKL/H-2Kb Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: CHO.SIINFEKL/H-2Kb
References identifiers: Patent=US20210380661;
References identifiers: PubMed=30347189;
Web pages: Provider; TTU; -; https://ttu.flintbox.com/technologies/48eb7349-649b-475b-85b9-759856124d72
Comments: Characteristics: Enables the large-scale and continuous batch production of soluble, fully folded and epitope-defined MHC class I molecules. Engineered to secrete peptide/MHC complexes that can be selectively purified in bulk using affinity chromatography, biotinylated, multimerized (via streptavidin) and the resulting reagent incorporated into immunologically relevant assays for detection of antigen-specific T-cells (PubMed=30347189).
Comments: Characteristics: Using a sleeping beauty transposon system a SIINFEKL-linker-B2m-linker-H2K(b)-AviTAge of donor at sampling:construct was integrated in the parent cell line (PubMed=30347189).
Comments: Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:88127; B2m.
Comments: Genetic integration: Method=Sleeping Beauty transposition; Gene=MGI; MGI:95904; H2-K1.
Comments: Genetic integration: Method=Sleeping Beauty transposition; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Comments: Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin: NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_E7G0
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® CHO-SIINFEKL/H-2Kb Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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