cho-k1/aequorin/ga16 GPCR稳定表达药物筛选用仓鼠卵巢（CHO-K1）细胞-BioVector NTCC典型培养物保藏中心

The NTCC® cho-k1/aequorin/ga16 cell line is a genetically engineered Chinese Hamster Ovary (CHO-K1) cell line used as a biosensor to measure changes in intracellular calcium levels. It is used in research and drug discovery to study G-protein coupled receptors (GPCRs) and other signaling pathways.

Components and function

• CHO-K1 host cells: A subclone of the original CHO cell line, CHO-K1 cells are a standard host for producing recombinant proteins and are easily transfected with foreign DNA. They are of Chinese hamster ovarian origin and require proline in their growth medium.

• Aequorin: A photoprotein that emits light in the presence of calcium. In the cho-k1/aequorin/ga16 line, the aequorin gene is stably expressed and targeted to a specific cellular location, such as the mitochondria, to measure localized calcium changes.

• Ga16: A chimeric G-protein alpha subunit that couples a wide range of receptors to the phospholipase C pathway, resulting in increased intracellular calcium. By co-expressing Ga16, the cell line can amplify the calcium response from various GPCRs.

• GPCR screening: Because the Ga16 protein couples with many GPCRs, the cell line can be used to screen for compounds that activate these receptors, making it a versatile tool in early-stage drug discovery.

• Calcium signaling studies: The aequorin reporter system allows for the detection of subtle or localized changes in intracellular calcium, which can provide insight into complex cellular signaling pathways.

• Mitochondrial calcium dynamics: In versions where aequorin is targeted to the mitochondria, the cell line can be used to specifically investigate mitochondrial calcium influx and its role in cell death and metabolism.

• CHO-K1宿主细胞： 标准的重组蛋白生产宿主细胞系，易于转染外源DNA。它源自中国仓鼠卵巢，需要在培养基中添加脯氨酸才能生长。

• 水母素（Aequorin）： 一种生物发光蛋白质，在钙离子存在时会发光。在 cho-k1/aequorin/ga16 细胞系中，水母素基因被稳定表达，并被靶向到特定的细胞位置（如线粒体），以测量局部钙离子的变化。

• Ga16： 一种嵌合G蛋白alpha亚基，可将各种受体与磷脂酶 C 通路偶联，从而导致细胞内钙离子增加。通过共表达 Ga16，该细胞系可以放大来自不同 GPCR 的钙离子反应。

• GPCR筛选： 由于 Ga16 蛋白与多种 GPCR 偶联，该细胞系可用于筛选激活这些受体的化合物，使其成为早期药物研发中一种多功能工具。

• 钙信号研究： 水母素报告系统能够检测细胞内钙离子细微或局部的变化，从而深入了解复杂的细胞信号通路。

• 线粒体钙动力学： 在水母素被靶向到线粒体的版本中，该细胞系可专门用于研究线粒体钙内流及其在细胞死亡和代谢中的作用。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net