UTI89尿路致病性大肠杆菌BIoVector®（UPEC）E.coli strain BioVector NTCC典型培养物保藏中心

价　　格：¥99850
货　　号：BIoVector®-UTI89
产　　地：北京

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BIoVector® UTI89 strain is a well-characterized, clinically isolated uropathogenic Escherichia coli (UPEC) used as a model organism in urinary tract infection (UTI) research. It was originally isolated from a patient with uncomplicated cystitis and has since been instrumental in studying the molecular mechanisms of UPEC virulence.

Key characteristics

Pathogenic origin: UTI89 is a clinical isolate from a human patient with acute bladder inflammation (cystitis). It belongs to phylogroup B2, a lineage of E. coli frequently associated with extraintestinal infections.
Virulence factors: UTI89 is equipped with several genetic features that enhance its ability to cause infection, including:
- Pathogenicity Islands (PAIs): It possesses four major PAIs, which are distinct from those in non-pathogenic E. coli strains and contain genes that contribute to its disease-causing potential.
- Virulence plasmid (pUTI89): This large extrachromosomal plasmid contains virulence genes and plays a critical role during the initial stages of infection by promoting bladder colonization and invasion.
- Adhesins: UTI89 has multiple pilus operons, including type 1 pili, which help it adhere to the urinary tract's epithelial cells. The FimH adhesin on the tip of type 1 pili is particularly important for this process.
- Toxins: The strain produces several toxins, including hemolysin and cytotoxic necrotizing factor (CNF), that can damage host cells and aid infection.
Infection cycle: In mouse models, UTI89 replicates within the bladder's urothelial cells, forming intracellular bacterial communities (IBCs). This provides a niche where the bacteria are protected from the host's immune response and antibiotics.

Research applications

The UTI89 strain's well-defined pathogenic features and sequenced genome make it a foundational tool for research into urinary tract infections. It is used to study:

UPEC fitness: Identifying the essential genes required for its survival and growth in different environments, including laboratory media, human urine, and the mouse bladder.
Host-pathogen interactions: Understanding how the bacterium adapts to the host's immune response and manipulates host cell processes.
Biofilm formation: Investigating the complex biofilm structures it creates at the air-liquid interface and how they protect the bacteria.
Therapeutic development: Testing novel strategies to combat antibiotic-resistant UPEC, such as phage therapy.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net

UTI89菌株是一种经过深入研究、从临床患者中分离得到的尿路致病性大肠杆菌（UPEC），被广泛用作尿路感染（UTI）研究的模型生物。它最初是从一名患有非复杂性膀胱炎的患者身上分离出来的。

主要特征

致病性来源： UTI89是从一名患有急性膀胱炎的患者身上分离出的临床菌株。它属于系统发育B2群，该谱系的大肠杆菌常与肠道外感染相关。
毒力因子： UTI89拥有多种增强其致病能力的遗传特征，其中包括：
- 致病岛（PAIs）：它拥有四个主要的致病岛，这些致病岛与非致病性大肠杆菌菌株不同，并包含有助于其致病潜力的基因。
- 毒力质粒（pUTI89）：这个大型染色体外质粒包含毒力基因，在感染的早期阶段通过促进膀胱定殖和侵袭而发挥关键作用。
- 黏附素： UTI89拥有多个菌毛操纵子，包括1型菌毛，这有助于它黏附到尿路的表皮细胞上。1型菌毛顶端的FimH黏附素对此过程尤为重要。
- 毒素：该菌株产生多种毒素，包括溶血素和细胞毒性坏死因子（CNF），这些毒素可以破坏宿主细胞并帮助感染。
感染周期：在小鼠模型中，UTI89在膀胱的尿路上皮细胞内复制，形成细胞内细菌群落（IBCs）。这为细菌提供了一个可以躲避宿主免疫反应和抗生素的“庇护所”。