EY917 NTCC™ 磷酸盐转运缺陷型酵母突变菌株-BioVector NTCC典型培养物保藏中心

NTCC™ EY917 酵母菌株是一种经过基因工程改造的酿酒酵母（Saccharomyces cerevisiae）突变菌株，专门用于磷酸盐转运研究。它是研究来自其他生物（特别是植物和真菌）的磷酸盐转运蛋白功能的关键工具。

主要特点

EY917 菌株的决定性特征是其磷酸盐转运系统有缺陷。这是通过敲除酵母基因组中所有已知的磷酸盐高亲和力和低亲和力转运蛋白基因来实现的。具体来说，该菌株缺失了五个关键的磷酸盐转运蛋白基因：

• pho84Δ

• pho87Δ

• pho89Δ

• pho90Δ

• pho91Δ

由于缺乏这些功能性转运蛋白，EY917 菌株无法有效地从环境中吸收无机磷酸盐（Pi）。这导致当其在低磷酸盐浓度的培养基中培养时，生长非常差。

研究应用

EY917 菌株的主要用途是进行互补性试验，以测试来自其他生物的基因是否能作为磷酸盐转运蛋白发挥作用。实验过程如下：

1. 将来自植物、真菌或其他生物的基因（候选基因）克隆到质粒中。

2. 然后将该质粒转化到 EY917 突变菌株中。

3. 在低磷酸盐培养基中培养转化的 EY917 细胞。

如果候选基因成功编码了一个功能性的磷酸盐转运蛋白，它将恢复酵母吸收磷酸盐的能力，使得 EY917 菌株能够正常生长或比未转化的菌株生长得更好。如果该基因不具有转运功能，酵母的生长将依然很差。这提供了一种直接且简单的方法，用于筛选和表征来自各种来源的磷酸盐转运蛋白基因的功能。

The EY917 yeast strain is a genetically engineered mutant strain of Saccharomyces cerevisiae (baker's yeast) that is specifically used in research on phosphate transport. It is a crucial tool for studying the function of phosphate transporters from other organisms, particularly plants and fungi.

Key Characteristics

The defining feature of the EY917 strain is that it has a defective phosphate transport system. This is achieved by deleting the genes for all known high-affinity and low-affinity phosphate transporters in the yeast genome. Specifically, the strain is a knockout for five key phosphate transporter genes:

• pho84Δ

  
  

• pho87Δ

  
  

• pho89Δ

  
  

• pho90Δ

• pho91Δ

Because it lacks these functional transporters, the EY917 strain is unable to effectively take up inorganic phosphate (Pi) from its environment. This results in very poor growth when cultured in media with low phosphate concentrations.

Research Applications

The primary use of the EY917 strain is in complementation assays to test whether a gene from another organism can function as a phosphate transporter. The experimental process works as follows:

1. A gene from a plant, fungus, or other organism (the candidate gene) is cloned into a plasmid.

2. This plasmid is then transformed into the EY917 mutant strain.

3. The transformed EY917 cells are grown in a low-phosphate medium.

If the candidate gene successfully encodes a functional phosphate transporter, it will restore the yeast's ability to absorb phosphate, allowing the EY917 strain to grow normally or grow significantly better than the untransformed strain. If the gene is not a functional transporter, the yeast will continue to show poor growth. This provides a direct and simple way to screen for and characterize the function of phosphate transporter genes from a wide variety of sources.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

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