NHEK正常人表皮角质形成细胞NTCC™（Normal Human Epidermal Keratinocytes）BioVector NTCC典型培养物保藏中心

NTCC™ NHEK cell stands for Normal Human Epidermal Keratinocytes. These are primary cells, meaning they are isolated directly from human skin tissue (typically from neonatal foreskins or adult skin) and maintained for a limited number of divisions in a lab. They are not a continuous cell line, which is immortalized and can replicate indefinitely.

Key Characteristics

• Primary Cells: NHEK cells are the closest in-vitro model to the cells found in human skin, making them highly relevant for physiological and biochemical studies.


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• Source: They are derived from the epidermis, which is the outermost layer of the skin. They are the most common cell type in the epidermis, making up about 90% of the cells.

• Differentiation: In the body, these cells originate in the basal layer of the epidermis and differentiate as they migrate upwards to the skin's surface, eventually forming the protective outer layer (stratum corneum). In a lab setting, they can be cultured to mimic this differentiation process.

• Limited Lifespan: Unlike immortalized cell lines, NHEK cells have a finite lifespan and will stop dividing after a certain number of passages (typically around 15-20 doublings).

Research Applications

NHEK cells are a fundamental tool in dermatological, toxicological, and cosmetic research because they accurately model the behavior of skin cells. They are used for:

• Skin Barrier Studies: Investigating how the skin maintains its protective barrier function against pathogens, chemicals, and water loss.

• Wound Healing: Studying the cellular processes involved in wound repair and testing the efficacy of wound care products.

• Toxicology and Safety Testing: Assessing the toxicity and irritancy of chemicals, drugs, and cosmetic ingredients on human skin.

• Dermatology: Modeling skin diseases like psoriasis and atopic dermatitis to understand their mechanisms and develop treatments.

• 3D Skin Models: NHEK cells are used to create complex 3D skin equivalent models, which closely resemble human skin tissue and serve as alternatives to animal testing.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net

NHEK 代表正常人表皮角质形成细胞（Normal Human Epidermal Keratinocytes）。它们是原代细胞，意味着它们直接从人体皮肤组织（通常是新生儿包皮或成人皮肤）中分离出来，并在实验室中进行有限次数的传代培养。它们不是永生化的连续细胞系，不能无限期地复制。

主要特点

• 原代细胞: NHEK 细胞是体外研究中最接近人体皮肤细胞的模型，因此在生理学和生物化学研究中具有高度相关性。

• 来源: 它们来自表皮，即皮肤的最外层。它们是表皮中最常见的细胞类型，约占细胞总数的90%。

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• 分化: 在体内，这些细胞起源于表皮的基底层，并随着向上迁移到皮肤表面而分化，最终形成保护性的外层（角质层）。在实验室环境中，可以通过培养来模拟这种分化过程。

• 有限寿命: 与永生化细胞系不同，NHEK 细胞的寿命有限，在经过一定次数的传代后（通常约15-20次倍增），它们会停止分裂。

研究应用

NHEK 细胞是皮肤病学、毒理学和化妆品研究的重要工具，因为它们能准确地模拟皮肤细胞的行为。它们被用于：

• 皮肤屏障研究: 探索皮肤如何维持其对病原体、化学物质和水分流失的保护性屏障功能。

• 伤口愈合: 研究伤口修复中涉及的细胞过程，并测试伤口护理产品的有效性。

• 毒理学与安全性测试: 评估化学品、药物和化妆品成分对人类皮肤的毒性和刺激性。

• 皮肤病学: 模拟银屑病和特应性皮炎等皮肤疾病，以了解其发病机制并开发治疗方法。

• 3D 皮肤模型: NHEK 细胞被用来构建复杂的3D 皮肤等效模型，这些模型与人体皮肤组织非常相似，可作为动物测试的替代方案。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net