出品公司: | Clontech |
---|---|
载体名称: | pLVX-TetOne-Luc |
质粒类型: | 慢病毒载体;四环素调控载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | TRE3GS, hPGK |
载体大小: | 9935 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
筛选标记: | 无 |
克隆菌株: | Stbl3 |
宿主细胞(系): | 常规细胞系(293、CV-1、CHO等) |
备注: | 慢病毒载体pLVX-TetOne-Luc是pLVX-TetOne的阳性对照载体。 |
产品目录号: | 631846 |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型 |
病毒/非病毒: | 慢病毒 |
pLVX-TetOne-Luc单质粒四环素诱导慢病毒Luc萤火虫酶荧光过表达载体-BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格:¥17930
- 货 号:pLVX-TetOne-Luc单质粒四环素诱导慢病毒Luc萤火虫酶荧光过表达载体
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
- 已注册
pLVX-TetOne-Luc vector
描述:
pLVX-TetOne-Luc单质粒四环素诱导慢病毒Luc萤火虫酶荧光过表达载体,hPGK启动子表达Tet-On 3G转录激活蛋白因子,TRE3GS启动子严谨型四环素调控启动子+Luciferase萤火虫酶荧光蛋白基因,Amp抗性。BioVector NTCC质粒载体菌种细胞基因保藏中心提供
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TetOne-Luc 9935 bp ds-DNA circular SYN 24-SEP-2013
COMMENT BioVector NTCC Inc. http://www.biovector.net
COMMENT 631844, 631845, 631846
FEATURES Location/Qualifiers
LTR 1..634
/note="5' LTR"
misc_feature 681..806
/note="Packaging signal"
misc_feature 1303..1536
/note="RRE"
misc_feature 2028..2144
/note="cPPT/CTS"
polyA_signal 2187..2321
/note="SV40 polyA signal"
CDS complement(2522..4174)
/label="luciferase"
/note="firefly luciferase"
promoter complement(4184..4548)
/note="TRE3GS promoter"
promoter 4568..5078
/note="hPGK promoter"
/note="human phosphoglycerate kinase 1 promoter"
CDS 5097..5843
/note="Tet-On 3G"
misc_feature 5857..6445
/note="WPRE"
LTR 6653..7286
/note="3' LTR"
rep_origin complement(7817..8402)
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8573..9433)
/note="AmpR"
promoter complement(9434..9538)
/note="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag
4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc
4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg
4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt
4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa
4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg
4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata
4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt
5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta
5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag
5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag
5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
5821 accttgacat gctccccggg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt
5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
6421 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
6481 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
6541 cacaagagga ggaggaggtg ggtttttcca gtcacacctc aggtaccttt aagaccaatg
6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg
6661 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
6721 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
6781 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
6841 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
6901 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
6961 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
7021 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
7081 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
7141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
7201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
7261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
7321 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac
7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
8341 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
9541 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca
9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
9721 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
9781 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc
9841 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat
9901 tgtatttgtt aaatatgtac tacaaactta gtagt
//
LOCUS pLVX-TetOne-Luc 9935 bp ds-DNA circular SYN 24-SEP-2013 COMMENT BioVector NTCC Inc. http://www.biovector.net COMMENT 631844, 631845, 631846 FEATURES Location/Qualifiers LTR 1..634 /note="5' LTR" misc_feature 681..806 /note="Packaging signal" misc_feature 1303..1536 /note="RRE" misc_feature 2028..2144 /note="cPPT/CTS" polyA_signal 2187..2321 /note="SV40 polyA signal" CDS complement(2522..4174) /label="luciferase" /note="firefly luciferase" promoter complement(4184..4548) /note="TRE3GS promoter" promoter 4568..5078 /note="hPGK promoter" /note="human phosphoglycerate kinase 1 promoter" CDS 5097..5843 /note="Tet-On 3G" misc_feature 5857..6445 /note="WPRE" LTR 6653..7286 /note="3' LTR" rep_origin complement(7817..8402) /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(8573..9433) /note="AmpR" promoter complement(9434..9538) /note="AmpR promoter" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat 2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg 2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg 2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca 2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat 2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg 2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg 2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca 2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg 2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa 2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag 2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga 2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa 2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga 3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct 3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg 3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat 3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa 3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa 3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca 3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg 3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa 3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat 3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa 3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta 3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca 3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc 3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac 3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca 3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca 3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg 4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc 4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg 4141 ccgggccttt ctttatgttt ttggcgtctt ccatggtgaa ttctttacga gggtaggaag 4201 tggtacggaa agttggtata agacaaaagt gttgtggaat tgaagtttac tcaaaaaatc 4261 agcactcttt tataggcgcc ctggtttaca taagcaaagc ttatacgttc tctatcactg 4321 atagggagta aactggatat acgttctcta tcactgatag ggagtaaact gtagatacgt 4381 tctctatcac tgatagggag taaactggtc atacgttctc tatcactgat agggagtaaa 4441 ctccttatac gttctctatc actgataggg agtaaagtct gcatacgttc tctatcactg 4501 atagggagta aactcttcat acgttctcta tcactgatag ggagtaaact cgaggtgata 4561 attccacggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg 4621 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct 4681 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga 4741 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt 4801 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa 4861 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg 4921 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc 4981 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg 5041 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatcatcga attaccatgt 5101 ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta 5161 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt 5221 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca 5281 ggcatcatac ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca 5341 acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg 5401 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc 5461 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac 5521 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta 5581 ccaccgattc tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag 5641 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa 5701 agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag 5761 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg 5821 accttgacat gctccccggg taaacgcgtc tggaacaatc aacctctgga ttacaaaatt 5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6241 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg 6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6361 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg 6421 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac 6481 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag 6541 cacaagagga ggaggaggtg ggtttttcca gtcacacctc aggtaccttt aagaccaatg 6601 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg 6661 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc 6721 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt 6781 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga 6841 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa 6901 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat 6961 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac 7021 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata 7081 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga 7141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 7201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 7261 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 7321 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgttttac 7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 8341 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 8401 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 8461 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 8521 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 8581 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 8641 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 8701 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 8761 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 8821 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 8881 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 8941 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 9001 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 9061 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 9121 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 9181 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 9241 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 9301 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 9361 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 9421 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 9481 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 9541 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca 9601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 9661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 9721 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc 9781 actgccaatt acctagtggt ttcatttact ctaaacctgt gattcctctg aattattttc 9841 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat 9901 tgtatttgtt aaatatgtac tacaaactta gtagt //
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