| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-TetOne-Puro-Luc |
| 质粒类型: | 慢病毒载体；四环素调控载体；荧光素酶报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TRE3GS , hPGK |
| 载体大小: | 10880 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 慢病毒载体pLVX-TetOne-Puro-Luc是荧光素酶报告载体，作为pLVX-TetOne-Puro载体的阳性对照。 |
| 产品目录号: | 631849 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
pLVX-TetOne-Puro-Luc-Control单质粒四环素诱导慢病毒Luc萤火虫酶荧光过表达载体-BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥17930
- 货 号：pLVX-TetOne-Puro-Luc-Control plasmid
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
pLVX-TetOne-Puro-Luc-Control plasmid
描述：
pLVX-TetOne-Puro-Luc-Control单质粒四环素诱导慢病毒Luc萤火虫酶荧光过表达载体Puro筛选标记，hPGK启动子表达Tet-On 3G转录激活蛋白因子，TRE3GS启动子严谨型四环素调控启动子+Luciferase萤火虫酶荧光蛋白基因，Puromycin嘌呤霉素细胞筛选标记，Amp抗性。BioVector NTCC质粒载体菌种细胞基因保藏中心提供
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-TetOne-Puro-Luc 10880 bp ds-DNA circular SYN 24-SEP-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Catalog Nos. 631847, 631848, 631849
FEATURES Location/Qualifiers
LTR 1..634
/label="5' LTR"
misc_feature 681..806
/label="Packaging signal"
misc_feature 1303..1536
/label="RRE"
misc_feature 2028..2144
/label="cPPT/CTS"
polyA_signal 2187..2321
/label="SV40 polyA signal"
CDS complement(2522..4174)
/label="luciferase"
/note="firefly luciferase"
promoter complement(4181..4545)
/label="TRE3GS promoter"
promoter complement(4565..5075)
/label="hPGK promoter"
CDS 5094..5840
/label="Tet-On 3G"
promoter 5851..6180
/label="SV40 promoter"
CDS 6189..6788
/label="PuroR"
misc_feature 6802..7390
/label="WPRE"
LTR 7598..8231
/label="3' LTR"
rep_origin complement(8762..9347)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9518..10378)
/codon_start=1
/label="AmpR"
promoter complement(10379..10483)
/label="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
4141 ccgggccttt ctttatgttt ttggcgtctt ccatgaattc tttacgaggg taggaagtgg
4201 tacggaaagt tggtataaga caaaagtgtt gtggaattga agtttactca aaaaatcagc
4261 actcttttat aggcgccctg gtttacataa gcaaagctta tacgttctct atcactgata
4321 gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct
4381 ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc
4441 cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata
4501 gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga ggtgataatt
4561 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
4621 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
4681 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
4741 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
4801 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
4861 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
4921 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
4981 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
5041 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta
5101 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
5161 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
5221 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
5281 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
5341 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
5401 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
5461 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
5521 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
5581 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
5641 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
5701 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
5761 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
5821 ttgacatgct ccccgggtaa acgcgcgaat gtgtgtcagt tagggtgtgg aaagtcccca
5881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
5941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
6001 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
6061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
6121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
6181 acgcgaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
6241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
6301 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
6361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
6421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
6481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
6541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
6601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
6661 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
6721 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
6781 gtgcctgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
7201 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
7321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
7381 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
7441 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
7501 aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
7561 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
7621 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
7681 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
7741 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
7801 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
7861 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
7921 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
7981 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
8041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
8101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
8161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
8221 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
8281 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
8341 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
8401 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
8461 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
8521 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
8581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
8641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
8701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
8761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
8821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
8881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
8941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
9001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
9061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
9121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
9181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
9241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
10441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
10501 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
10561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
10621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
10681 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
10741 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
10801 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
10861 tgtactacaa acttagtagt
//
LOCUS pLVX-TetOne-Puro-Luc 10880 bp ds-DNA circular SYN 24-SEP-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Catalog Nos. 631847, 631848, 631849
FEATURES Location/Qualifiers
LTR 1..634
/label="5' LTR"
misc_feature 681..806
/label="Packaging signal"
misc_feature 1303..1536
/label="RRE"
misc_feature 2028..2144
/label="cPPT/CTS"
polyA_signal 2187..2321
/label="SV40 polyA signal"
CDS complement(2522..4174)
/label="luciferase"
/note="firefly luciferase"
promoter complement(4181..4545)
/label="TRE3GS promoter"
promoter complement(4565..5075)
/label="hPGK promoter"
CDS 5094..5840
/label="Tet-On 3G"
promoter 5851..6180
/label="SV40 promoter"
CDS 6189..6788
/label="PuroR"
misc_feature 6802..7390
/label="WPRE"
LTR 7598..8231
/label="3' LTR"
rep_origin complement(8762..9347)
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9518..10378)
/codon_start=1
/label="AmpR"
promoter complement(10379..10483)
/label="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc catcgataga tctgcggccg
2521 cttacaattt ggactttccg cccttcttgg cctttatgag gatctctctg atttttcttg
2581 cgtcgagttt tccggtaaga cctttcggta cttcgtccac aaacacaact cctccgcgca
2641 actttttcgc ggttgttact tgactggcga cgtaatccac gatctctttt tccgtcatcg
2701 tctttccgtg ctccaaaaca acaacggcgg cgggaagttc accggcgtca tcgtcgggaa
2761 gacctgccac gcccgcgtcg aagatgttgg ggtgttgtaa caatatcgac tccaattcag
2821 cgggggccac ctggtatcct ttgtatttaa ttaaagactt caagcggtca actatgaaga
2881 agtgttcgtc ttcgtcccag taagctatgt ctccagaatg tagccatcca tccttgtcaa
2941 tcaaggcgtt ggtcgcttcc ggattgttta cataaccgga cataatcata ggtcctctga
3001 cacataattc gcctctctga ttaacgccca gcgttttccc ggtatccaga tccacaacct
3061 tcgcttcaaa aaatggaaca actttaccga ccgcgcccgg tttatcatcc ccctcgggtg
3121 taatcagaat agctgatgta gtctcagtga gcccatatcc ttgtcgtatc cctggaagat
3181 ggaagcgttt tgcaaccgct tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa
3241 tttcgtgtaa attagataaa tcgtatttgt caatcagagt gcttttggcg aagaatgaaa
3301 atagggttgg tactagcaac gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca
3361 gctcttcttc aaatctatac attaagacga ctcgaaatcc acatatcaaa tatccgagtg
3421 tagtaaacat tccaaaaccg tgatggaatg gaacaacact taaaatcgca gtatccggaa
3481 tgatttgatt gccaaaaata ggatctctgg catgcgagaa tctgacgcag gcagttctat
3541 gcggaagggc cacaccctta ggtaacccag tagatccaga ggagttcatt atcagtgcaa
3601 ttgttttgtc acgatcaaag gactctggta caaaatcgta ttcattaaaa ccgggaggta
3661 gatgagatgt gacgaacgtg tacatcgact gaaatccctg gtaatccgtt ttagaatcca
3721 tgataataat tttctggatt attggtaatt ttttttgcac gttcaaaatt ttttgcaacc
3781 cctttttgga aacaaacact acggtaggct gcgaaatgtt catactgttg agcaattcac
3841 gttcattata aatgtcgttc gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca
3901 ccggcataaa gaattgaaga gagttttcac tgcatacgac gattctgtga tttgtattca
3961 gcccatatcg tttcatagct tctgccaacc gaacggacat ttcgaagtat tccgcgtacg
4021 tgatgttcac ctcgatatgt gcatctgtaa aagcaattgt tccaggaacc agggcgtatc
4081 tcttcatagc cttatgcagt tgctctccag cggttccatc ctcaagagga tagaatggcg
4141 ccgggccttt ctttatgttt ttggcgtctt ccatgaattc tttacgaggg taggaagtgg
4201 tacggaaagt tggtataaga caaaagtgtt gtggaattga agtttactca aaaaatcagc
4261 actcttttat aggcgccctg gtttacataa gcaaagctta tacgttctct atcactgata
4321 gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta gatacgttct
4381 ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg gagtaaactc
4441 cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct atcactgata
4501 gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga ggtgataatt
4561 ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
4621 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
4681 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
4741 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
4801 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
4861 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
4921 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
4981 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
5041 ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt accatgtcta
5101 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
5161 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
5221 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
5281 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
5341 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
5401 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
5461 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
5521 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
5581 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
5641 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
5701 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
5761 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
5821 ttgacatgct ccccgggtaa acgcgcgaat gtgtgtcagt tagggtgtgg aaagtcccca
5881 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
5941 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
6001 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
6061 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
6121 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
6181 acgcgaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
6241 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
6301 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
6361 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
6421 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
6481 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
6541 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
6601 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
6661 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
6721 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
6781 gtgcctgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact
6841 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
6901 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
6961 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
7021 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
7081 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
7141 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
7201 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
7261 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
7321 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
7381 gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa
7441 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
7501 aggtgggttt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
7561 gtagatctta gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa
7621 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
7681 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
7741 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
7801 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
7861 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
7921 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
7981 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
8041 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
8101 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
8161 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
8221 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
8281 atgaatatca gagagtgaga ggccttgaca ttgctagcgt tttaccgtcg acctctagct
8341 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
8401 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
8461 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
8521 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
8581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
8641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
8701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
8761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
8821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
8881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
8941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
9001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
9061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
9121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
9181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
9241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt
9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
10441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
10501 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
10561 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
10621 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
10681 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattaccta
10741 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
10801 tatttgttaa atatgtacta caaacttagt agtttttaaa gaaattgtat ttgttaaata
10861 tgtactacaa acttagtagt
//
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