HEK293 Actinin-M-cpstFRET人转化细胞株NTCC® Human Transformed cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID HEK293 Actinin-M-cpstFRET Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HEK293 M-cpstFRET
Cross-references:Kerafast; EBU024
Cross-references:Wikidata; Q98126661
References identifiers:PubMed=25422450;
Comments:Characteristics: Used to report forces in actinin in living cells. Has one actinin molecule with the cpstFRET sensor incorporated between spectrin repeat domains 3 and 4 toward the middle of actinin. The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Comments:Genetic integration: Method=Transfection; Gene=HGNC; HGNC:163; ACTN1.
Comments:Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments:Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Comments:Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin:NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy:CVCL_0045 ! HEK293
Sex of cell:Female
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC® CVCL_ZV89
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培养方法：
Medium培养基：NTCC® ID HEK293 Actinin-M-cpstFRET Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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