KM13辅助噬菌体 BioVector NTCC质粒载体菌种细胞基因保藏中心

KM13辅助噬菌体

Description:

KM13 phage contains a protease cleavage sitebetween the second and third domains of their minor coat protein pIII,rendering the phage without fusion protein non-infective at the trypsin elutionstep of biopanning.

KM13 is a popular helperphage that allows for elution using trypsin (although my experience suggeststhat elution be performed using TEA first, then trypsin treatment). Althoughthis cuts the displayed protein, the genetic information is still containedwithin the phage. Once you infect bacteria with the eluted phage and amplify,the protein becomes displayed on the coat protein of the amplified phage forsubsequent rounds of panning.

Source:
KM13 phage supernatant was isolated from infectedE. coliER2738 by astandard procedure.

BioVector NTCC质粒载体菌种细胞基因保藏中心