BJ2168 yeast strain蛋白酶缺陷型酵母菌菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BJ2168酵母菌株

BioVector NTCC质粒载体菌种细胞基因保藏中心

BJ2168 strain

Cat# BioVector503343  

Product category

Fungi

Product type

Yeast

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Strain designation

BJ2168

Type strain

No

Ploidy

Haploid

Genotype

MATa prc1-407 prb1-1122 pep4-3 leu2 trp1 ura3-52 gal2

Comments

Proteinase deficient (CH1)

Homozygous prb1 mutations alter sporulation; homozygous pep4 prevent it.

Medium

YPAD medium

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen. If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week. Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C). Storage of frozen material at this temperature will result in the death of the culture.

1.To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

2.Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.

3.Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

Handling notes

-Proteinase deficient (CH1)

-Homozygous prb1 mutations alter sporulation; homozygous pep4 prevent it.