pSGP18 BioVector®毕赤酵母CBP和His标签表达载体 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pSGP18 BioVector®毕赤酵母CBP和His标签表达载体

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pSGP18 is a yeast expression vector. Here's a breakdown of its key features:

• Yeast Expression: It's designed for protein expression in the yeast Pichia pastoris.

• AOX1 Promoter: This promoter drives strong gene expression in Pichia pastoris when induced with methanol.

• C-terminal Tags:

  • CBP (Calmodulin Binding Protein): Allows for affinity purification of the expressed protein using calmodulin affinity chromatography.

  • 6xHis-tag: Enables protein purification using nickel-nitrilotriacetic acid (Ni-NTA) affinity chromatography.

  • 3C Protease Cleavage Site: Allows for the removal of the CBP and 6xHis-tags after purification.

  
  

• Zeocin Resistance: Confers resistance to the antibiotic zeocin, allowing for selection of yeast cells that have successfully integrated the plasmid.

• Ligation-Independent Cloning (LIC): This method simplifies the process of inserting genes into the vector.

Key Applications:

• Heterologous Protein Expression: pSGP18 is widely used to express recombinant proteins in Pichia pastoris. This yeast system offers several advantages, including high protein yields, proper protein folding and post-translational modifications, and ease of cultivation.

• Protein Purification: The CBP and 6xHis-tags facilitate efficient purification of the expressed protein using affinity chromatography.

• Protein Characterization: The expressed protein can be further characterized for its biochemical and functional properties.

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