Nalm6-Fluc-Puro/HuCD19-Puro (mid)人类癌细胞株NTCC® Human Cancer cell line细胞系-BioVector NTCC细胞保藏中心
- 价 格：¥99850
- 货 号：NTCC®-E4BU
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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[Designation名称] NTCC® ID Nalm6-Fluc-Puro/HuCD19-Puro (mid) Cell Line细胞株
BioVector NTCC典型培养物保藏中心Cross-references: Imanis; CL183Comments: Population: Caucasian.Comments: Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).Comments: Characteristics: Transduced with a human CD19 and PuroR encoding lentivirus. A moderate CD19 expressing population was generated by selection using a methylcellulose based semi-solid medium (Imanis=CL183).Comments: Sequence variation: Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).Comments: Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC®-CVCL_E4BU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID Nalm6-Fluc-Puro/HuCD19-Puro (mid) Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC典型培养物保藏中心Cross-references: Imanis; CL183Comments: Population: Caucasian.Comments: Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).Comments: Characteristics: Transduced with a human CD19 and PuroR encoding lentivirus. A moderate CD19 expressing population was generated by selection using a methylcellulose based semi-solid medium (Imanis=CL183).Comments: Sequence variation: Gene fusion; HGNC; HGNC:50800; DUX4 + HGNC; HGNC:5477; IGH; Name(s)=IGH-DUX4; Note=The translocation occurs in the silenced IGH allele (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:3236; EGFR; Simple; p.Arg1068Ter (c.3202C>T); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Ala146Thr (c.436G>A); Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:9864; RARA; Simple; p.Gln45Ter (c.133C>T); ClinVar=VCV000393019; Zygosity=Heterozygous (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:1633; CD19.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).Comments: Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC®-CVCL_E4BU
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID Nalm6-Fluc-Puro/HuCD19-Puro (mid) Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
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