K562-Fluc-Puro/HuBCMA-Neo人类癌细胞株NTCC® Human Cancer cell line细胞系-BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID K562-Fluc-Puro/HuBCMA-Neo Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Cross-references: Imanis; CL192
Comments: Population: Caucasian.
Comments: Characteristics: Reporter cell line, transduced with the LV-Fluc-P2A-PuroR vector encoding for luciferase linked via a P2A cleavage peptide to the PuroR gene and under control of the spleen focus-forming virus (SFFV) promoter (from parent cell line).
Comments: Characteristics: Transduced with a human TNFRS17/BCMA and neo encoding lentivirus (Imanis=CL192).
Comments: Sequence variation: Gene fusion; HGNC; HGNC:76; ABL1 + HGNC; HGNC:1014; BCR; Name(s)=BCR-ABL1, BCR-ABL; Note=BCR exon 14 fused to ABL1 exon 2 (b3a2 transcript) (from parent cell line).
Comments: Sequence variation: Mutation; HGNC; HGNC:11998; TP53; Simple; p.Gln136fs*13 (c.406_407insC); Zygosity=Homozygous (from parent cell line).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:11913; TNFRSF17.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Comments: Derived from site: In situ; Pleural effusion; UBERON=UBERON_0000175.
Diseases: NCIt; C9110; Blast phase chronic myelogenous leukemia, BCR-ABL1 positive
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession Number: NTCC®-CVCL_E4BI
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID K562-Fluc-Puro/HuBCMA-Neo Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net