pSEVA321 BioVector®低拷贝广宿主质粒载体 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pSEVA321低拷贝广宿主质粒载体

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pSEVA321 is a member of the Standard European Vector Architecture (SEVA) collection of plasmids. These plasmids are designed to be highly standardized and compatible, facilitating genetic engineering and synthetic biology research.

pSEVA321 plasmid map

Key Features:

• Origin of Replication (RK2): Allows the plasmid to replicate in a broad range of Gram-negative bacteria.

• Chloramphenicol Resistance Gene (CmR): Confers resistance to the antibiotic chloramphenicol, enabling selection of bacteria carrying the plasmid.

• Multiple Cloning Site (MCS): Contains a variety of restriction enzyme recognition sites, allowing for the insertion of foreign DNA fragments.

• Standardized Structure: Adheres to the SEVA architecture, promoting compatibility and interchangeability of genetic parts.

Applications:

• Gene cloning and expression: Introducing foreign genes into a wide range of Gram-negative bacteria.

• Synthetic biology: Constructing genetic circuits and pathways for various applications.

• Biotechnology: Developing new tools and technologies for industrial and medical applications.

Advantages:

• Broad Host Range: Replicates in many Gram-negative bacteria.

• Standardization: The SEVA architecture ensures compatibility and interchangeability of genetic parts.

• Versatile cloning: The MCS allows for flexible insertion of DNA fragments.

• Well-characterized: The properties of SEVA plasmids are well-documented, facilitating their use in research.

Limitations:

• Limited copy number: The copy number of pSEVA321 in some bacteria may be relatively low, which can limit protein expression levels.

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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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