pCI-neo-mOVA BioVector®膜结合卵清蛋白哺乳动物表达质粒 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pCI-neo-mOVA BioVector®膜结合卵清蛋白哺乳动物表达质粒

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pCI-neo-mOVA is a mammalian expression vector designed to express membrane-bound ovalbumin (mOVA) in mammalian cells. It is a valuable tool for research in immunology, cancer biology, and vaccine development.

pCIneomOVA plasmid map

Key Features:

• Strong promoter (CMV): The human cytomegalovirus (CMV) immediate-early enhancer/promoter drives high levels of gene expression in mammalian cells.

• Neomycin resistance gene: Confers resistance to the antibiotic G418, allowing for selection of transfected cells.

• Membrane-bound ovalbumin (mOVA): A model antigen commonly used in immunology studies.

Applications:

• Immunology research: Studying antigen presentation, T cell responses, and vaccine development.

• Cancer research: Investigating tumor immunology and developing cancer immunotherapies.

• Vaccine development: Designing and testing subunit vaccines against infectious diseases and cancer.

How it Works:

1. Transfection: The pCI-neo-mOVA plasmid is introduced into mammalian cells using methods like transfection or electroporation.

2. Gene expression: The CMV promoter drives the expression of mOVA mRNA.

3. Protein synthesis: The mRNA is translated into mOVA protein, which is targeted to the cell membrane.

4. Antigen presentation: The mOVA protein is processed and presented on the cell surface by MHC molecules, stimulating T cell responses.


mOVA protein on cell membrane

Advantages:

• High expression levels: The CMV promoter ensures strong expression of mOVA.

• Easy selection: The neomycin resistance gene allows for efficient selection of transfected cells.

• Well-characterized antigen: mOVA is a widely studied antigen with well-defined immune responses.

Limitations:

• Potential for insertional mutagenesis: Random integration of the plasmid into the host genome can disrupt cellular genes.

• Transient expression: In some cases, the plasmid may be lost over time, leading to decreased mOVA expression.

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生产厂家Supplier:

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail:BioVector@163.com

http://www.biovector.net