NSO NTCC®小鼠骨髓瘤细胞株单抗生产用细胞mouse myeloma cell line BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

NSO NTCC®小鼠骨髓瘤细胞株单抗生产用细胞mouse myeloma cell line

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

The NSO cell line is a mouse myeloma cell line, widely used in biotechnology and monoclonal antibody production. It is particularly valuable in the generation of hybridomas and the production of recombinant proteins, including therapeutic monoclonal antibodies.

Key Features of the NSO Cell Line:

1. Origin:
   The NSO cell line is derived from a mouse myeloma (cancerous B cell) that is used as a host cell line in hybridoma technology. Myeloma cells like NSO are typically used in fusion with mouse spleen cells to create hybridomas, which are capable of producing large amounts of a specific monoclonal antibody.

2. Characteristics:

   • Immortalized: The NSO cell line is immortalized, meaning it can divide indefinitely in culture, which is critical for large-scale production of antibodies.

   • Lack of Immunoglobulin Production: One of the key features of NSO cells is that they do not produce endogenous immunoglobulins. This trait is important because it allows for the production of monoclonal antibodies without interference from the cell’s own antibodies, making them an ideal host for recombinant antibody production.

   • High Yield: NSO cells are known for their ability to produce high-yield recombinant proteins and are often used in large-scale production of monoclonal antibodies.

3. Applications:

   • Monoclonal Antibody Production: The NSO cell line is extensively used in the production of monoclonal antibodies for diagnostic and therapeutic purposes. They serve as the base cells in hybridoma technology, where they are fused with B cells from an immunized mouse to produce hybridoma cells that produce a specific monoclonal antibody.

   • Biotechnological Production: NSO cells are engineered to produce recombinant proteins, including therapeutic proteins such as cytokines, hormones, and enzymes.

   • Gene Expression: Researchers use NSO cells to express recombinant genes, enabling the large-scale production of proteins for research, drug development, and commercial purposes.

4. Transfection and Genetic Modification:
   NSO cells are also commonly transfected with recombinant DNA constructs to produce proteins of interest. Their ability to produce large quantities of proteins makes them valuable for protein engineering and drug discovery.

5. Adaptability for Suspension Culture:
   In addition to being adherent in culture, NSO cells can also be cultured in suspension, which is useful for large-scale bioreactor processes in industrial biotechnology, where high-density cell cultures are required for efficient protein production.

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