pRW50 BioVector®原核表达载体 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pRW50 BioVector®原核表达载体

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

The pRW50 plasmid is a type of vector commonly used in molecular biology for gene expression and genetic manipulation. It is often used to facilitate the cloning, expression, or modification of genes in bacterial systems, typically Escherichia coli (E. coli), and sometimes for other types of cells or organisms.

Here are the main characteristics and uses of the pRW50 plasmid:

Key Features of the pRW50 Plasmid:

1. Vector Type:
   The pRW50 plasmid is a bacterial expression plasmid. It's typically used in E. coli for cloning and protein expression purposes.

2. Origin of Replication:
   pRW50 usually contains an origin of replication (such as the pMB1 or ColE1 origin) that allows the plasmid to replicate independently in bacterial cells, ensuring the propagation of the plasmid and its contents.

3. Selectable Marker:
   The plasmid often contains a selectable marker, such as an antibiotic resistance gene (commonly for ampicillin or kanamycin resistance), enabling the selection of bacterial cells that have successfully taken up the plasmid by growing them in the presence of the corresponding antibiotic.

4. Cloning Sites:
   It features multiple cloning sites (MCS), which are short sequences that contain a variety of restriction enzyme recognition sites. These allow easy insertion of foreign DNA into the plasmid for gene cloning or expression experiments.

5. Gene Expression Control:
   Some versions of the pRW50 plasmid may have promoters that allow for the inducible expression of the cloned gene, depending on the specific version of the plasmid. The most commonly used promoters for this purpose include the lac promoter, which can be induced with IPTG (Isopropyl β-D-1-thiogalactopyranoside), or T7 promoters in cases where T7 RNA polymerase is provided in the system.

6. Applications:

   • Gene Cloning: The plasmid can be used to insert genes of interest for cloning purposes. After insertion, the plasmid can be introduced into bacteria, which will propagate the recombinant plasmid.

   • Protein Expression: pRW50 is also used for protein expression in bacterial cells. Once the gene of interest is inserted into the plasmid, the bacteria can be induced to produce the protein encoded by that gene.

   • Mutagenesis and Functional Studies: This plasmid can be used in site-directed mutagenesis or for functional studies of proteins, as well as for expressing recombinant proteins that may be purified for further research.

7. Plasmid Size and Capacity:

   • The size of pRW50 is moderate (typically between 5 to 10 kb), allowing it to carry and express relatively large inserts.

   • The plasmid is designed to accommodate foreign DNA without significantly affecting its replication and expression in bacteria.

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