pACNR1180 BioVector®病毒反向遗传载体长基因克隆质粒 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pACNR1180 BioVector®病毒反向遗传载体长基因克隆质粒

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pACNR1180 is a low-copy number plasmid derived from pACYC177, a commonly used plasmid in molecular biology.

Key Features:

• Low Copy Number: This characteristic is crucial for maintaining the stability of large DNA inserts, such as those used in cloning full-length viral genomes. High copy number plasmids can be unstable in bacteria, leading to deletions or rearrangements of the inserted DNA.

  
  

• Origin of Replication: pACNR1180 likely retains the origin of replication from pACYC177, which ensures its replication within bacterial cells.

• Multiple Cloning Site (MCS): It likely contains a multiple cloning site (MCS), a region with various restriction enzyme recognition sites, allowing for the easy insertion of foreign DNA fragments.

• Antibiotic Resistance Gene: pACNR1180 likely carries an antibiotic resistance gene, such as ampicillin resistance, which enables the selection of bacteria that have successfully taken up the plasmid.

Applications:

• Cloning Large DNA Inserts:Due to its low copy number, pACNR1180 is particularly suitable for cloning and maintaining large DNA fragments, such as full-length viral genomes or large genomic DNA fragments.

  
  

• Reverse Genetics: It can be used in reverse genetics approaches to generate infectious viruses from cloned cDNA.

  
  

• Gene Expression: While not typically used for high-level gene expression, it can be used to express genes at moderate levels in bacterial cells.

Significance:

pACNR1180 and other low-copy number plasmids are valuable tools in molecular biology, enabling researchers to work with and manipulate large DNA fragments that would be unstable in high-copy number plasmids. This has significant implications for various research areas, including virology, genetics, and biotechnology.

Map

Sequence: Contact BioVector NTCC Inc.

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