pMuc2-luc BioVector®基因荧光报告质粒plasmid BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pMuc2-luc BioVector®基因荧光报告质粒

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pMuc2-luc is a reporter plasmid designed to study the regulation of the MUC2 gene.

• MUC2: This gene encodes mucin 2, a major component of the mucus layer that lines the gastrointestinal tract. MUC2 plays a crucial role in protecting the intestinal epithelium from damage and infection.

• Reporter Plasmid: These plasmids contain a gene that encodes a readily detectable protein, such as luciferase, under the control of a specific promoter.

Structure of pMuc2-luc:

• MUC2 Promoter: The plasmid likely contains a fragment of the MUC2 gene promoter region upstream of the luciferase gene.

• Luciferase Gene (luc): This gene encodes luciferase, an enzyme that produces light in the presence of its substrate (luciferin).

• Other Elements: The plasmid may also contain other elements such as:

  • Origin of Replication: Enables the plasmid to replicate in bacteria.

  • Antibiotic Resistance Gene: Allows for selection of bacteria containing the plasmid.

  
  

Applications of pMuc2-luc:

• Studying MUC2 Gene Regulation:

  • Researchers can use pMuc2-luc to investigate the factors that regulate MUC2 gene expression.

  • This can involve analyzing the effects of different stimuli (e.g., cytokines, growth factors, hormones) on luciferase activity, which reflects MUC2 promoter activity.

  
  

• Identifying Regulatory Elements:

  • By deleting or mutating specific regions of the MUC2 promoter in the plasmid, researchers can identify important regulatory elements that control MUC2 gene expression.

  
  

• Drug Discovery:

  • pMuc2-luc can be used to screen for compounds that modulate MUC2 gene expression, which may have therapeutic potential in diseases involving abnormal mucus production (e.g., inflammatory bowel disease, cystic fibrosis).

  
  

Note: The specific design and components of pMuc2-luc may vary depending on the laboratory and research objectives.

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