pHH21 BioVector®反向遗传质粒用于流感病毒基因克隆操作 BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pHH21 BioVector®反向遗传质粒用于流感病毒基因克隆操作

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pHH21 is a widely used reverse genetics plasmid specifically designed for cloning and manipulating influenza virus genes.

pHH21 plasmid map

Key Features:

• High-Copy Origin of Replication: Contains an origin of replication that allows for high-copy number replication in bacteria, ensuring ample plasmid DNA for subsequent steps.

• Influenza Gene Segment Cloning Sites: Features unique cloning sites for each of the eight influenza virus gene segments (PB2, PB1, PA, HA, NP, NA, M, NS).

• Polymerase I Promoter: Includes a polymerase I promoter sequence, which is essential for transcribing the cloned influenza gene segments into viral RNA within the host cell.

• Ampicillin Resistance Gene: Contains the ampR gene, conferring resistance to the antibiotic ampicillin, enabling selection of bacteria harboring the plasmid.

Applications:

• Influenza Virus Reverse Genetics: Used as a backbone for generating recombinant influenza viruses from cloned cDNA.

• Influenza Virus Gene Manipulation: Enables the introduction of specific mutations or modifications into influenza virus genes.

• Influenza Virus Research: Used in various research areas, including vaccine development, pathogenesis studies, and antiviral drug discovery.

Significance:

pHH21 and its derivatives have revolutionized influenza virus research. By enabling the generation of recombinant viruses with precise genetic modifications, these plasmids have provided invaluable tools for understanding influenza virus biology and developing effective countermeasures.

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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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