人原代永生化髓核细胞系NTCC® Human Primary Immortalized Nucleus Pulposus Cell Line BioVector NTCC Inc.

NTCC®人原代永生化髓核细胞系

Human Primary Immortalized Nucleus Pulposus Cell Line
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Introduction

Human primary immortalized nucleus pulposus cell lines are derived from human intervertebral disc nucleus pulposus tissue and have been specially treated to acquire unlimited proliferation capacity. These cell lines are of great significance in research areas such as intervertebral disc degeneration, regenerative medicine, and drug screening.

Origin and Preparation

• Origin: Isolated from human intervertebral disc nucleus pulposus tissue.

• Immortalization: Immortalized by transfecting specific genes (such as SV40 T antigen, hTERT, etc.) or viral infection to acquire unlimited proliferation capacity.


Isolation process of human primary nucleus pulposus cells

Characteristics

• Morphology: Diverse morphology, can be spindle-shaped, polygonal, or star-shaped.

• Markers: Express nucleus pulposus cell-specific markers, such as type II collagen, Aggrecan, etc.

• Function: Retain some biological characteristics of primary nucleus pulposus cells, such as synthesizing extracellular matrix and secreting growth factors.

• Proliferation capacity: Have unlimited proliferation capacity under in vitro culture conditions.

Applications

• Intervertebral disc degeneration research: Study the molecular mechanisms of intervertebral disc degeneration and screen therapeutic drugs.

• Regenerative medicine: Used in intervertebral disc regeneration engineering research to construct tissue-engineered intervertebral discs.

• Drug screening: Screen drugs for the treatment of intervertebral disc diseases.

• Cell therapy: Explore the possibility of cell therapy for intervertebral disc degeneration.

Advantages

• Unlimited proliferation: Provide a stable cell source for large-scale experiments.

• Uniformity: Cells are derived from a single source, reducing experimental errors.

• Strong operability: Easy to perform genetic manipulation and drug screening.

Challenges

• Phenotypic drift: Long-term culture may lead to changes in cell phenotype, affecting experimental results.

• Risk of tumor formation: Immortalized cells have a potential risk of tumor formation.

• Not fully representative of primary cells: The immortalization process may lead to changes in cell function, which does not fully represent all the characteristics of primary nucleus pulposus cells.

Future Outlook

Human primary immortalized nucleus pulposus cell lines have broad application prospects in the research of intervertebral disc diseases. With the continuous development of technology, it is believed that these cell lines will play an increasingly important role in the field of intervertebral disc regenerative medicine.

Intervertebral disc degeneration diagram

Tissueengineered intervertebral disc

Precautions

• Choose appropriate immortalization methods to ensure the biological characteristics of the cells.

• Establish a strict quality control system to ensure cell purity and stability.

• During the experiment, pay attention to the cell passage number to avoid changes in cell phenotype due to long-term passage.

Keywords: Human primary cells, immortalization, nucleus pulposus cells, intervertebral disc degeneration, regenerative medicine, drug screening

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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail:BioVector@163.com

http://www.biovector.net