pHAGE TRE dCas9-KRAB BioVector®哺乳动物慢病毒基因编辑质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
- 价 格:¥39850
- 货 号:BioVector® pHAGE TRE dCas9-KRAB
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
电话:400-800-2947 工作QQ:1843439339 (微信同号)
邮件:Biovector@163.com
手机:18901268599
地址:北京
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pHAGE TRE dCas9-KRAB BioVector®哺乳动物慢病毒基因编辑质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
BioVector® pHAGE TRE dCas9-KRAB
Understanding the Components:
pHAGE: This likely refers to a lentiviral vector system, a common tool for delivering genetic material into cells. Lentiviruses can integrate their genetic material into the host cell's genome, allowing for stable and long-term expression.
TRE: This stands for Tetracycline Response Element. It's a DNA sequence that can be activated or repressed by the presence or absence of tetracycline or its derivatives, such as doxycycline. This allows for inducible gene expression.
dCas9: A deactivated form of the Cas9 protein. While it can still bind to specific DNA sequences guided by a single-guide RNA (sgRNA), it lacks the ability to cut DNA.
KRAB: A transcriptional repressor domain. When fused to dCas9, it can effectively silence gene expression by binding to the target DNA sequence and recruiting other proteins to repress transcription.
How it Works:
Lentiviral Delivery: The pHAGE vector containing the TRE-dCas9-KRAB construct is packaged into lentiviral particles.
Cell Infection: These particles infect target cells, and the viral DNA integrates into the host genome.
Inducible Expression: In the absence of doxycycline, the TRE promoter is inactive, and dCas9-KRAB is not expressed.
Gene Repression: When doxycycline is added, it binds to a regulatory protein, activating the TRE promoter. This leads to the expression of dCas9-KRAB.
Target Gene Silencing: The dCas9-KRAB complex binds to specific DNA sequences targeted by sgRNAs, leading to transcriptional repression of the target gene.
Applications:
Gene Function Studies: Silencing genes to study their role in cellular processes.
Disease Modeling: Creating cellular models of diseases by repressing specific genes involved in disease pathogenesis.
Therapeutic Development: Identifying potential drug targets by silencing genes and observing phenotypic effects.
Genome Engineering: Precisely controlling gene expression in a temporal and spatial manner.
Key Advantages:
Inducible Expression: Allows for precise control of gene repression.
High Specificity: The CRISPR-Cas9 system enables highly specific targeting of genes.
Potent Repression: The KRAB domain is a powerful transcriptional repressor.
Versatile Platform: Can be used in a variety of cell types and organisms.
生产厂家Supplier:
BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
E-mail:BioVector@163.com
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