首页 » pHAGE TRE dCas9-KRAB BioVector®哺乳动物慢病毒基因编辑质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pHAGE TRE dCas9-KRAB BioVector®哺乳动物慢病毒基因编辑质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

  • 价  格:¥39850
  • 货  号:BioVector® pHAGE TRE dCas9-KRAB
  • 产  地:北京
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pHAGE TRE dCas9-KRAB BioVector®哺乳动物慢病毒基因编辑质粒-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

BioVector® pHAGE TRE dCas9-KRAB

Understanding the Components:

  • pHAGE: This likely refers to a lentiviral vector system, a common tool for delivering genetic material into cells. Lentiviruses can integrate their genetic material into the host cell's genome, allowing for stable and long-term expression.

  • TRE: This stands for Tetracycline Response Element. It's a DNA sequence that can be activated or repressed by the presence or absence of tetracycline or its derivatives, such as doxycycline. This allows for inducible gene expression.

  • dCas9: A deactivated form of the Cas9 protein. While it can still bind to specific DNA sequences guided by a single-guide RNA (sgRNA), it lacks the ability to cut DNA.

  • KRAB: A transcriptional repressor domain. When fused to dCas9, it can effectively silence gene expression by binding to the target DNA sequence and recruiting other proteins to repress transcription.

How it Works:

  1. Lentiviral Delivery: The pHAGE vector containing the TRE-dCas9-KRAB construct is packaged into lentiviral particles.

  2. Cell Infection: These particles infect target cells, and the viral DNA integrates into the host genome.

  3. Inducible Expression: In the absence of doxycycline, the TRE promoter is inactive, and dCas9-KRAB is not expressed.

  4. Gene Repression: When doxycycline is added, it binds to a regulatory protein, activating the TRE promoter. This leads to the expression of dCas9-KRAB.

  5. Target Gene Silencing: The dCas9-KRAB complex binds to specific DNA sequences targeted by sgRNAs, leading to transcriptional repression of the target gene.

Applications:

  • Gene Function Studies: Silencing genes to study their role in cellular processes.

  • Disease Modeling: Creating cellular models of diseases by repressing specific genes involved in disease pathogenesis.

  • Therapeutic Development: Identifying potential drug targets by silencing genes and observing phenotypic effects.

  • Genome Engineering: Precisely controlling gene expression in a temporal and spatial manner.

Key Advantages:

  • Inducible Expression: Allows for precise control of gene repression.

  • High Specificity: The CRISPR-Cas9 system enables highly specific targeting of genes.

  • Potent Repression: The KRAB domain is a powerful transcriptional repressor.

  • Versatile Platform: Can be used in a variety of cell types and organisms.

Addgene:pHAGE TRE dCas9-KRAB

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