pAAV2/9n BioVector®腺相关病毒包装质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

pAAV2/9n BioVector®腺相关病毒包装质粒载体-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

AAV packaging plasmid expressing Rep/Cap genes

The pAAV2/9n plasmid is a valuable tool in molecular biology and gene therapy research. It is used to package adeno-associated virus (AAV) serotype 9, a popular choice for gene delivery due to its broad tissue tropism and low immunogenicity.

pAAV2/9n plasmid structure

Key features of pAAV2/9n plasmid:

• AAV2 backbone: Provides the basic structure and replication machinery for AAV production.

• Serotype 9 capsid: Encodes the capsid proteins responsible for AAV9's unique tissue targeting properties.

• Multiple cloning sites (MCS): Allows for the insertion of your desired gene or transgene of interest.

• Selection markers: Typically include antibiotic resistance genes (e.g., ampicillin) for easy selection of transformed bacteria.

Applications of pAAV2/9n plasmid:

• Gene therapy: Delivering therapeutic genes to specific tissues for treatment of genetic disorders.

• Basic research: Studying gene function and expression in various cell types.

• Vaccine development: Developing novel vaccines against infectious diseases.

Important considerations:

• Production of AAV vectors: The pAAV2/9n plasmid is typically used in conjunction with helper plasmids to produce infectious AAV vectors.

• Safety: AAV vectors are generally considered safe, but it is important to follow proper safety procedures when working with them.

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