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IncP RK2 BioVector®大肠杆菌、铜绿假单胞菌和克雷伯氏肺炎菌等广宿主穿梭质粒载体 BioVector NTCC保藏中心

  • 价  格:¥59850
  • 货  号:BioVector®IncP RK2
  • 产  地:北京
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IncP RK2 BioVector®大肠杆菌、铜绿假单胞菌克雷伯氏肺炎菌等广宿主穿梭质粒载体

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心


IncP RK2 Plasmid: A Versatile Genetic Tool

The IncP RK2 plasmid is a remarkable piece of DNA that has captivated the attention of microbiologists and genetic engineers alike. Its unique properties and wide host range make it a valuable tool for various applications in biotechnology and research.



Key Characteristics:

  • Broad Host Range: RK2 can replicate and transfer itself between a wide range of Gram-negative bacteria, including Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. This versatility makes it a powerful tool for genetic engineering experiments.

  • Self-Transmissible: RK2 carries genes that enable it to transfer itself from one bacterium to another through conjugation, a process involving direct cell-to-cell contact. This property allows for rapid dissemination of genetic information.

  • Antibiotic Resistance: RK2 often carries genes conferring resistance to multiple antibiotics, such as ampicillin, kanamycin, and tetracycline. These resistance genes can be used as selectable markers in genetic engineering experiments.

  • Complex Regulatory Network: RK2 possesses a sophisticated regulatory system that controls its replication, transfer, and maintenance. This system ensures that the plasmid is stably inherited by daughter cells and efficiently transferred to new hosts.

Applications:

  • Genetic Engineering: RK2-based vectors are widely used to introduce foreign genes into bacteria. Its broad host range allows for genetic manipulation of a variety of bacterial species.

  • Molecular Biology Research: RK2 plasmids are valuable tools for studying plasmid replication, transfer, and gene expression.

  • Biotechnology: RK2 can be used to express recombinant proteins in bacteria, making it a useful tool for industrial biotechnology.

The IncP RK2 plasmid continues to be a valuable asset in the field of microbiology and biotechnology. Its unique properties and versatility have made it an indispensable tool for researchers and scientists worldwide.

IncP RK2 质粒:一种多功能遗传工具

IncP RK2 质粒是一种引人注目的 DNA 片段,吸引了微生物学家和遗传工程师的广泛关注。其独特的特性和广泛的宿主范围使其成为生物技术和研究领域各种应用的有价值工具。

[IncP RK2 质粒的图谱]

主要特征:

  • 广泛的宿主范围: RK2 可以在多种革兰氏阴性细菌之间复制和转移自身,包括 大肠杆菌铜绿假单胞菌克雷伯氏肺炎菌。这种多功能性使其成为遗传工程实验的强大工具。

  • 自传递性: RK2 携带基因,使其能够通过接合(一种涉及直接细胞间接触的过程)将自身从一个细菌转移到另一个细菌。这种特性允许快速传播遗传信息。

  • 抗生素抗性: RK2 通常携带赋予多种抗生素抗性的基因,如氨苄青霉素、卡那霉素和四环素。这些抗性基因可用作遗传工程实验中的选择标记。

  • 复杂的调控网络: RK2 拥有一个复杂的调控系统,控制其复制、转移和维持。该系统确保质粒被子细胞稳定遗传并有效转移到新宿主。

应用:

  • 遗传工程: 基于 RK2 的载体广泛用于将外源基因引入细菌。其广泛的宿主范围允许对多种细菌物种进行基因操作。

  • 分子生物学研究: RK2 质粒是研究质粒复制、转移和基因表达的有价值工具。

  • 生物技术: RK2 可用于在细菌中表达重组蛋白,使其成为工业生物技术的有用工具。

IncP RK2 质粒继续成为微生物学和生物技术领域的重要资产。其独特的特性和多功能性使其成为全球研究人员和科学家不可或缺的工具。


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BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

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