OUMS-23 NTCC®从转移性心包液中建立的人结肠癌细胞株human colon carcinoma cell line-BioVector NTCC保藏中心
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- 货 号:NTCC®-1022 OUMS-23
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OUMS-23 NTCC®从转移性心包液中建立的人结肠癌细胞株human colon carcinoma cell line-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
Cell Name | OUMS-23 | ||
---|---|---|---|
Profile | A human colon carcinoma cell line established from metastatic pericardial fluid. | Other Name | |
Animal | human | Strain | |
Genus | Homo | Species | sapiens |
Sex | Age | ||
Identity | available | Tissue for Primary Cancer | colon |
Case history | familial adenomatous polyposis patient with colon cancer | Metastasis | yes |
Tissue Metastasized | pericardial fluid | Genetics | Expression of the APC gene decreased to one-half compared with the normal fibroblast cells. No mutaion at codons 12 and 61 of H-, K-, and N-ras proto oncogenes. |
Life Span | Crisis PDL | ||
Morphology | epithelial-like | Character | Cells express heat-stable placental-type APase and carcinoembryonic antigen. Chromosomes are between 53 and 118. Cells can grow in soft agar at cloning efficiency of 6.5%. The cells form tumors in the nude mice diagnosed as differentiated adenocarcinoma. |
Classify | tumor | Established by | Miyazaki,M. & Namba,M. |
Registered by | Namba,M. | Regulation for Distribution | |
Comment | Mycoplasmas decontaminated in the JCRB Cell Bank. | Year | 2002 |
Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum. | Methods for Passages | Cells were treated with 0.1% trypsin and 0.02% EDTA when harvesting cells. |
Cell Number on Passage | Race | ||
CO2 Conc. | 5 % | Tissue Sampling | colon |
Tissue Type |
Detection of virus genome fragment by Real-time PCR | |||||||||
---|---|---|---|---|---|---|---|---|---|
Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
HHV7 | - | HTLV-2 | - | -/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) | |||||
BKV | - | HIV-1 | - | ||||||
JCV | - | HIV-2 | - | ||||||
ADV | - | HPV18 | - | ||||||
Notes |
Link | |
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Cellosaurus(ExPASy) | CVCL_3088 |
Reference | |
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Pubmed id:8576285 | Establishment and characterization of a human colon cancer cell line, OUMS-23, from a patient with familial adenomatous polyposis. Miyazaki M,Tsuboi S,Mihara K,Kosaka T,Fukaya K,Kino K,Mori M,Namba M J Cancer Res Clin Oncol. 1996;122(2):95-101 |
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