KMM-1人源多发性骨髓瘤细胞株Lambda-链生成NTCC®-BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
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NTCC® KMM-1人源多发性骨髓瘤细胞株 | ||||
Comment | Human myeloma. Lambda- chain producing. |
Basic information | Depositor | Huh, Nam, Ho | ||
Originator | Namba, Masayoshi | |||
Year of deposit | 1987 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Race | Japanese | |||
Gender | Male | |||
Age at sampling | 62 years | |||
Tissue | myeloma, subcutaneous | |||
Disease name | multiple myeloma | |||
Metastatic ability | Yes | |||
Metastatic tissue | Multiple | |||
Classification | cancer | |||
Year of origin | 1980 | |||
History | Namba,M.->RCB | |||
Memo_1 | Mycoplasma was eliminated with MC-210. | |||
Lifespan | infinite | |||
Morphology | lymphocyte-like | |||
Cellosaurus(Expasy) | CVCL_2981 | |||
Medium | ||||
Culture type | Suspension cells | |||
Culture method | ||||
Culture medium | RPMI1640 + 10% FBS | |||
Antibiotics | Free | |||
Passage method | dilution | |||
Culture information | Passage ratio | 1 : 10 split | ||
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma/Acholeplasma | (-) | |||
Virus (HIV) | (-) | |||
Isozyme | LD, NP | |||
Chromosome mode | 38-166(50) : /77(4),78(4),79(4),80(5),81(4),82(3)/ | |||
STR(human) | OK |
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