HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3人类转化细胞株NTCC® Human Transformed cell line-BioVector NTCC细胞保藏中心CVCL_D4BX

[Designation名称] NTCC® HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: Syn10 Galectin-3 FRET
Cross references: Wikidata; Q127381683
Comments: Characteristics: Transduced with an all-in-one TetOff construct for the controllable expression of untagged SNCA p.Ala53Thr (from parent cell line).
Comments: Characteristics: Additional expression of two plasmids encoding mRuby3- and mClover3-tagged LGALS3, enabling a FRET-based readout of endolysosome damage (Direct_author_submission).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; 11138; SNCA (Note=With p.Ala53Thr).
Comments: Genetic integration: Method=Transduction; Gene=HGNC; 6563; LGALS3.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; Q8ISF8; Entacmaea quadricolor eqFP611 (modified to produce the bright RFP mRuby3).
Comments: Genetic integration: Method=Transduction; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_D4BX
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK293T TetOff-SNCA-A53T mRuby3-Galectin-3 mClover3-Galectin-3 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net