HEK293-DR-GFP-RAD51C-2/C+人类转化细胞株NTCC® Human Transformed cell line-BioVector NTCC细胞保藏中心CVCL_XX07
- 价 格：¥99850
- 货 号：NTCC®-XX07
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作QQ:1843439339 (微信同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
[Designation名称] NTCC® HEK293-DR-GFP-RAD51C-2/C+ Cell Line细胞株
BioVector NTCC典型培养物保藏中心Cross references: DSMZ; ACC-851Cross references: DSMZCellDive; ACC-851Cross references: Wikidata; Q94095054Comments: Characteristics: The parental RAD51C KO cell line is functionally reconstituted by retroviral transfection of RAD51C.Comments: Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.Comments: Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.Comments: Doubling time: ~30 hours (Note=After thawing), ~10-20 hours (Note=Later passages) (DSMZ=ACC-851).Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; 9820; RAD51C.Comments: Genetic integration: Method=Transfection/transduction; Gene=HGNC; 9820; RAD51C.Comments: Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_XX07
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK293-DR-GFP-RAD51C-2/C+ Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC典型培养物保藏中心Cross references: DSMZ; ACC-851Cross references: DSMZCellDive; ACC-851Cross references: Wikidata; Q94095054Comments: Characteristics: The parental RAD51C KO cell line is functionally reconstituted by retroviral transfection of RAD51C.Comments: Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.Comments: Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.Comments: Doubling time: ~30 hours (Note=After thawing), ~10-20 hours (Note=Later passages) (DSMZ=ACC-851).Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; 9820; RAD51C.Comments: Genetic integration: Method=Transfection/transduction; Gene=HGNC; 9820; RAD51C.Comments: Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_XX07
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® HEK293-DR-GFP-RAD51C-2/C+ Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
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