HEK293-DR-GFP-RAD51C-2人类转化细胞株NTCC® Human Transformed cell line-BioVector NTCC细胞保藏中心CVCL_XX02

[Designation名称] NTCC® HEK293-DR-GFP-RAD51C-2 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Cross references: DSMZ; ACC-846
Cross references: DSMZCellDive; ACC-846
Cross references: Wikidata; Q94095050
Comments: Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
Comments: Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Comments: Doubling time: ~40 hours (DSMZ=ACC-846).
Comments: Knockout cell: Method=CRISPR/Cas9; HGNC; 9820; RAD51C.
Comments: Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
Comments: Transformant: NCBI_TaxID; 28285; Adenovirus 5.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_XX02
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® HEK293-DR-GFP-RAD51C-2 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net