HEK293 cpstFRET-Actin人类转化细胞株NTCC® Human Transformed cell line-BioVector NTCC细胞保藏中心CVCL_ZV86

[Designation名称] NTCC® HEK293 cpstFRET-Actin Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HEK293 cpA; Beta-C-HEK
Cross references: Kerafast; EBU023
Cross references: Wikidata; Q98126662
Reference Identifiers: PubMed=25422450;
Comments: Characteristics: Has one actin molecule attached to the C-terminal of cpstFRET, leaving the other end of the probe dangling free and the probe devoid of tension (force-free control). The cpstFRET (circularly permuted stretch sensitive FRET) sensor consists of a pair of tandemly connected circularly permutated CFP/YFPs (cpCerulean/cpVenus). Mechanical loading twists the dipole orientation from near parallel to diagonal, decreasing FRET efficiency.
Comments: Genetic integration: Method=Transfection; Gene=HGNC; 132; ACTB.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments: Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Comments: Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_0045 ! HEK293
Sex of cell: Female
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-Accession: CVCL_ZV86
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® HEK293 cpstFRET-Actin Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net