CRISPRi Gen1B 1人类诱导多能干细胞NTCC® Human Induced pluripotent stem cell细胞系-BioVector NTCC细胞保藏中心CVCL_VM35

[Designation名称] NTCC® CRISPRi Gen1B 1 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

SY WTB CRISPRi Gen1 clone 1; 1B-1
DR Wikidata; Q93458758
RX PubMed=26971820;
CC Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
CC Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
CC Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
CC Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
OX NCBI_TaxID=9606; ! Homo sapiens (Human)
HI CVCL_VM30 ! WTB6
SX Female
AG Age unspecified
CA Induced pluripotent stem cell
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_VM35
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® CRISPRi Gen1B 1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net