CHO-K1-CNG-Gs中国仓鼠自发永生化细胞株NTCC® Chinese hamster Spontaneously immortalized cell line细胞系-BioVector NTCC细胞保藏中心CVCL_C0RV

[Designation名称] NTCC® CHO-K1-CNG-Gs Cell Line细胞株
BioVector NTCC典型培养物保藏中心

SY CHO-K1-CNG
DR Wikidata; Q112929380
CC Characteristics: Expresses a modified cyclic nucleotide gated (CNG) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. Allows sensitive detection of signaling by Gs, Gi or Gq-coupled receptors (Codex BioSolutions).
CC Characteristics: Expresses a constitutively active over-expressed Gs-coupled GPCR. Serves as a parent cell line for various ACTOne PDE cell lines.
CC Genetic integration: Method=Transfection/transduction; Gene=RGD; 2367; Cnga2 (Note=With p.Phe68Ala, p.Trp81Ala, p.Cys460Trp and p.Glu583Met = oCNG-NC).
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC Miscellaneous: Has Codex Biosolutions catalog number CB-81300-100, but not distributed separately from children cell lines.
CC Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI CVCL_0214 ! CHO-K1
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C0RV
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® CHO-K1-CNG-Gs Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net