AE17小鼠间皮细胞瘤细胞株NTCC® Mouse Mesothelioma Cell Line BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® AE17小鼠间皮细胞瘤细胞株NTCC® Mouse Mesothelioma Cell Line

AE17 Cell Line
Cat No.: NTCC®922826

Cell Line Name
AE17

Cell Line Description
Mice (C57Black/6J strain female 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice.

Tissue of Origin
Lung

Applications
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Disease
Mesothelioma

Cell Type
Epithelial-like

Image


Subculture Routine
Optimal split ratio 1:16 - 1:32 (seeding density 0.6 x10⁴ cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Culture Medium
RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Growth Mode
Adherent

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net