hNav1.8-HEK293电压门控钠通道α亚基稳转表达细胞株NTCC® Recombinant Stable Cell Line BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® NTCC® hNav1.8-HEK293电压门控钠通道α亚基稳转表达细胞株NTCC® Recombinant Stable Cell Line

NTCC® hNav1.8 Recombinant Stable Cell Line
Catalog Number  NTCC®-NAV1.8SCLot NumberSee Vial

Contents1 Flask, 1 x 106 to 2 x 106 cells

Background Information
hNav1.8 is a voltage-gated sodium channel alpha subunit. It is expressed in mammalian adult dorsal root ganglion (DRG) neurons, predominantly those with small diameter cell bodies that give rise to the unmyelinated (C-type) fibres. These fibres form synapses in the dorsal horn of the spinal cord and are mainly involved in pain signaling. Additional information can be found on page 2.

Product Information
DescriptionRecombinant HEK 293 cell line expressing the human Nav1.8 (tetrodotoxin-resistant voltage-gated sodium channel type X, SCN10A)

FamilySodium, Voltage-Gated
TargetNav1.8

Target ProteinAccession Number
1Nav1.8AF117907

SpeciesHuman
Host Cell TypeHEK 293
ApplicationElectrophysiology assay (conventional and automated patch clamp platforms)
StorageVials are to be stored in vapor phase of liquid nitrogen

Functional Performance
HEK293 cells expressing hNav1.8 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques.

Electrophysiology MethodQPatch
Reference Agonist
Reference AntagonistTetracaine
Antagonist IC50 ( M)0.57








1

Passage Stability
This cell line has been confirmed to be stable through at least 12 passages with no significant drop in assay window or change in pharmacology.


Mycoplasma Testing
This lot was tested and found to be free of mycoplasma contamination. Data available upon request.



Notes
Additional functional (pharmacological and electrophysiological) validation on multiple platforms is available upon request.



Additional Ligand Information Control CompoundTetracaine Vendor Name :Sigma-Aldrich Vendor Catalog No. T7383

Additional Background Information
This channel conducts the characteristic slow, tetrodotoxin-resistant (TTX-r) currents involved in action potential initiation and transmission in these neurons and is therefore a potential target for analgesic agents. Altered expression of Nav1.8 is observed in pain models and human pain states - channel protein can be seen to re-localise from cell bodies to the site of insult or injury. There is also good evidence from antisense oligonucleotide studies for a role in development of hyperexciteable states in pain models. However KO mice show surprisingly limited pain phenotypes, possibly due to compensatory up-regulation of other Nav subtypes.









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NTCC®-NAV1.8SC

NTCC® hNav1.8 Recombinant Stable Cell Line

Generated on : June 29, 20202


Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net